Detailed information of TA system TA182654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 7916..8573
• Replicon: plasmid pMrsPuff_01
• Accession: NZ_CP065439
• Organism: Klebsiella pneumoniae strain Mrs. Puff

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: I5M55_RS26955
• Protein ID: WP_000270043.1
• Coordinates: 7916..8266 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5M55_RS26960
• Protein ID: WP_000124640.1
• Coordinates: 8271..8573 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M55_RS26920	4390..4887	-	498	WP_000062185.1	hypothetical protein	-
I5M55_RS26925	4890..5378	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
I5M55_RS26930	5475..5810	+	336	WP_000683476.1	hypothetical protein	-
I5M55_RS26935	5825..6295	-	471	WP_001281821.1	hypothetical protein	-
I5M55_RS26940	6288..6659	-	372	WP_000516916.1	hypothetical protein	-
I5M55_RS26945	6670..6864	-	195	WP_000343597.1	hypothetical protein	-
I5M55_RS26950	7205..7753	-	549	WP_001061574.1	transcriptional regulator	-
I5M55_RS26955	7916..8266	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5M55_RS26960	8271..8573	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
I5M55_RS26965	8600..8893	-	294	WP_001239997.1	chromosome segregation protein ParM	-
I5M55_RS26970	8981..9253	-	273	WP_001043047.1	HU family DNA-binding protein	-
I5M55_RS26975	9311..9838	-	528	WP_001236377.1	thermonuclease family protein	-
I5M55_RS26980	10069..10926	-	858	WP_001167032.1	hypothetical protein	-
I5M55_RS26985	10913..11143	-	231	WP_000972663.1	hypothetical protein	-
I5M55_RS26990	11143..11661	-	519	WP_000210756.1	nitrite reductase	-
I5M55_RS26995	11658..12104	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
I5M55_RS27000	12104..12463	-	360	WP_000422768.1	hypothetical protein	-
I5M55_RS27005	12520..12948	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / rmtB / erm(42)	-	1..148704	148704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T182654 WP_000270043.1 NZ_CP065439:7916-8266 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T182654 NZ_CP086526:2025261-2025364 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT182654 WP_000124640.1 NZ_CP065439:8271-8573 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT182654 NZ_CP086526:c2025402-2025131 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9