Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4351535..4352181 Replicon chromosome
Accession NZ_CP065436
Organism Klebsiella pneumoniae strain Dirty Dan

Toxin (Protein)


Gene name higB Uniprot ID A0A4S7KZ03
Locus tag I6Z21_RS21140 Protein ID WP_032433636.1
Coordinates 4351535..4351882 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag I6Z21_RS21145 Protein ID WP_019725405.1
Coordinates 4351882..4352181 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6Z21_RS21130 4347461..4348894 + 1434 WP_002920564.1 glycogen synthase GlgA -
I6Z21_RS21135 4348912..4351359 + 2448 WP_004202135.1 glycogen phosphorylase -
I6Z21_RS21140 4351535..4351882 + 348 WP_032433636.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6Z21_RS21145 4351882..4352181 + 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
I6Z21_RS21150 4352244..4353752 - 1509 WP_029602548.1 glycerol-3-phosphate dehydrogenase -
I6Z21_RS21155 4353957..4354286 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
I6Z21_RS21160 4354337..4355167 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
I6Z21_RS21165 4355217..4355975 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13500.51 Da        Isoelectric Point: 6.2327

>T182632 WP_032433636.1 NZ_CP065436:4351535-4351882 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGINEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T182632 NZ_CP086520:767283-767579 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT182632 WP_019725405.1 NZ_CP065436:4351882-4352181 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT182632 NZ_CP086520:767581-767976 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S7KZ03

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References