Detailed information of TA system TA182625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2562885..2563482
• Replicon: chromosome
• Accession: NZ_CP065436
• Organism: Klebsiella pneumoniae strain Dirty Dan

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: I6Z21_RS12590
• Protein ID: WP_004893639.1
• Coordinates: 2563165..2563482 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: I6Z21_RS12585
• Protein ID: WP_004142561.1
• Coordinates: 2562885..2563172 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6Z21_RS12555	2558965..2559213	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
I6Z21_RS12560	2559231..2559572	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
I6Z21_RS12565	2559603..2560718	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
I6Z21_RS12570	2560898..2561482	+	585	WP_002893026.1	TetR/AcrR family transcriptional regulator	-
I6Z21_RS12575	2561479..2561847	+	369	WP_002893024.1	MmcQ/YjbR family DNA-binding protein	-
I6Z21_RS12580	2561967..2562620	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
I6Z21_RS12585	2562885..2563172	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
I6Z21_RS12590	2563165..2563482	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6Z21_RS12595	2563667..2564710	-	1044	WP_194428733.1	DUF2157 domain-containing protein	-
I6Z21_RS12600	2565376..2566242	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
I6Z21_RS12605	2566351..2567778	+	1428	WP_023300814.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T182625 WP_004893639.1 NZ_CP065436:c2563482-2563165 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T182625 NZ_CP086513:c68272-67970 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT182625 WP_004142561.1 NZ_CP065436:c2563172-2562885 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT182625 NZ_CP086513:c67899-67561 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1