Detailed information of TA system TA182615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3202..3787
• Replicon: plasmid pAB17961-2
• Accession: NZ_CP065434
• Organism: Acinetobacter baumannii strain ATCC 17961

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: I5593_RS19370
• Protein ID: WP_000897309.1
• Coordinates: 3467..3787 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5593_RS19365
• Protein ID: WP_000369782.1
• Coordinates: 3202..3474 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5593_RS19345	1..924	+	924	WP_000845851.1	replication initiation protein RepM	-
I5593_RS19350	992..1537	+	546	WP_000736774.1	plasmid replication DNA-binding protein	-
I5593_RS19355	1765..2196	-	432	WP_000042163.1	MarR family transcriptional regulator	-
I5593_RS19360	2203..2631	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
I5593_RS19365	3202..3474	-	273	WP_000369782.1	helix-turn-helix domain-containing protein	Antitoxin
I5593_RS19370	3467..3787	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5593_RS19375	3974..4171	-	198	WP_000476222.1	hypothetical protein	-
I5593_RS19380	4238..4465	-	228	WP_000921865.1	hypothetical protein	-
I5593_RS19385	4563..4778	-	216	WP_000071892.1	cold-shock protein	-
I5593_RS19390	5109..5300	-	192	WP_000102616.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6667	6667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T182615 WP_000897309.1 NZ_CP065434:c3787-3467 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T182615 NZ_CP086510:3792682-3792960 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10064.50 Da
• Isoelectric Point: 7.2045

>AT182615 WP_000369782.1 NZ_CP065434:c3474-3202 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPGLI

Nucleotide

• Length: 273 bp

>AT182615 NZ_CP086510:3792419-3792679 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3