Detailed information of TA system TA182614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4282..4933
• Replicon: plasmid pAB17961-1
• Accession: NZ_CP065433
• Organism: Acinetobacter baumannii strain ATCC 17961

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: I5593_RS19320
• Protein ID: WP_000269904.1
• Coordinates: 4577..4933 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5593_RS19315
• Protein ID: WP_001129974.1
• Coordinates: 4282..4584 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5593_RS19280	1..936	+	936	WP_001292329.1	replication initiation protein RepM	-
I5593_RS19285	1014..1532	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
I5593_RS19290	1772..2203	-	432	WP_000042163.1	MarR family transcriptional regulator	-
I5593_RS19295	2210..2638	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
I5593_RS19300	2836..3348	-	513	WP_001089573.1	SMI1/KNR4 family protein	-
I5593_RS19305	3387..3905	-	519	WP_000447196.1	sel1 repeat family protein	-
I5593_RS19310	4080..4289	-	210	WP_000069474.1	hypothetical protein	-
I5593_RS19315	4282..4584	-	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
I5593_RS19320	4577..4933	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5593_RS19325	5551..5979	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
I5593_RS19330	5986..6417	+	432	WP_000042163.1	MarR family transcriptional regulator	-
I5593_RS19335	6564..7325	-	762	WP_000425105.1	hypothetical protein	-
I5593_RS19340	7630..8799	+	1170	WP_002134206.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9395	9395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T182614 WP_000269904.1 NZ_CP065433:c4933-4577 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T182614 NZ_CP086510:c3786620-3786222 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT182614 WP_001129974.1 NZ_CP065433:c4584-4282 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT182614 NZ_CP086510:c3786916-3786623 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: N8YFZ1