Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5870411..5871006 Replicon chromosome
Accession NZ_CP065417
Organism Pseudomonas aeruginosa isolate P23

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag I5U72_RS27320 Protein ID WP_003113526.1
Coordinates 5870728..5871006 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I5U72_RS27315 Protein ID WP_003099268.1
Coordinates 5870411..5870716 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5U72_RS27285 5865457..5865891 - 435 WP_009875803.1 hypothetical protein -
I5U72_RS27290 5866407..5866697 - 291 WP_152959653.1 DUF5447 family protein -
I5U72_RS27295 5866873..5867145 - 273 WP_058175553.1 hypothetical protein -
I5U72_RS27300 5867592..5867927 + 336 WP_058175552.1 hypothetical protein -
I5U72_RS27305 5867930..5868631 - 702 WP_152959654.1 retron Ec48 family effector membrane protein -
I5U72_RS27310 5868618..5869793 - 1176 WP_071557852.1 reverse transcriptase family protein -
I5U72_RS27315 5870411..5870716 - 306 WP_003099268.1 HigA family addiction module antidote protein Antitoxin
I5U72_RS27320 5870728..5871006 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I5U72_RS27325 5871335..5873563 + 2229 WP_003099265.1 TonB-dependent receptor -
I5U72_RS27330 5873633..5874280 - 648 WP_003095021.1 carbonate dehydratase -
I5U72_RS27335 5874342..5875580 - 1239 WP_003113524.1 dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-97)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T182590 WP_003113526.1 NZ_CP065417:c5871006-5870728 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T182590 NZ_CP086502:c59297-59148 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11213.79 Da        Isoelectric Point: 5.0747

>AT182590 WP_003099268.1 NZ_CP065417:c5870716-5870411 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT182590 NZ_CP086502:59341-59402 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References