Detailed information of TA system TA182581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5264590..5265332
• Replicon: chromosome
• Accession: NZ_CP065411
• Organism: Kosakonia radicincitans strain IAC-BECa95

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I5960_RS25385
• Protein ID: WP_035942746.1
• Coordinates: 5265021..5265332 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5960_RS25380
• Protein ID: WP_007372402.1
• Coordinates: 5264590..5265024 (-)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5960_RS25370	5262171..5263520	+	1350	WP_007372404.1	lysine-sensitive aspartokinase 3	-
I5960_RS25375	5263614..5264555	+	942	WP_007372403.1	ketopantoate/pantoate/pantothenate transporter PanS	-
I5960_RS25380	5264590..5265024	-	435	WP_007372402.1	helix-turn-helix domain-containing protein	Antitoxin
I5960_RS25385	5265021..5265332	-	312	WP_035942746.1	type II toxin-antitoxin system HigB family toxin	Toxin
I5960_RS25390	5265582..5265854	+	273	WP_007372400.1	DUF3811 domain-containing protein	-
I5960_RS25395	5265855..5266718	-	864	WP_007372399.1	23S rRNA pseudouridine(2604) synthase RluF	-
I5960_RS25400	5266853..5268484	-	1632	WP_007372398.1	Na/Pi cotransporter family protein	-
I5960_RS25405	5268659..5269126	-	468	WP_227136188.1	hypothetical protein	-
I5960_RS25410	5269110..5269664	-	555	WP_227136189.1	hypothetical protein	-
I5960_RS25415	5269664..5269918	-	255	WP_227136190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (23-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12272.11 Da
• Isoelectric Point: 9.9087

>T182581 WP_035942746.1 NZ_CP065411:c5265332-5265021 [Kosakonia radicincitans]
MHVISRRPFDSAANDFPNHALVLDAVYKVLKSTSYRNPDELREEFPSLDRMKYRDKWWVINIAGNHLRMLFFADFGAGKI
FVKHIVTHAEYDKLVKRYRETKE

Nucleotide

• Length: 312 bp

>T182581 NZ_CP086500:2755704-2755811 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 16013.09 Da
• Isoelectric Point: 4.5542

>AT182581 WP_007372402.1 NZ_CP065411:c5265024-5264590 [Kosakonia radicincitans]
MIDDAIKAADQLVKQVPFLGNRPSREDYEHALAMVEQLLEQAPDSPLVELLAAKIERYEDNDPELVAFNTRVASLPRGVA
ALRVLMDQYGLNQSSFGHEIGQRSLVSRILNGERNLTVDHIRALAERFNVSTDVFIEPVSQTAG

Nucleotide

• Length: 435 bp

>AT182581 NZ_CP086500:c2755657-2755591 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT