Detailed information of TA system TA182574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3235028..3235744
• Replicon: chromosome
• Accession: NZ_CP065411
• Organism: Kosakonia radicincitans strain IAC-BECa95

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I5960_RS15785
• Protein ID: WP_227136875.1
• Coordinates: 3235421..3235744 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5960_RS15780
• Protein ID: WP_227135717.1
• Coordinates: 3235028..3235363 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5960_RS15750	3230921..3231937	-	1017	WP_227135711.1	tail fiber protein	-
I5960_RS15755	3232010..3232351	-	342	WP_227135712.1	hypothetical protein	-
I5960_RS15760	3232300..3232719	-	420	WP_227135713.1	hypothetical protein	-
I5960_RS15765	3232722..3233099	-	378	WP_227135714.1	hypothetical protein	-
I5960_RS15770	3233126..3233365	-	240	WP_227135715.1	hypothetical protein	-
I5960_RS15775	3234068..3234589	-	522	WP_227135716.1	hypothetical protein	-
I5960_RS15780	3235028..3235363	-	336	WP_227135717.1	HigA family addiction module antitoxin	Antitoxin
I5960_RS15785	3235421..3235744	-	324	WP_227136875.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5960_RS15790	3235958..3236275	-	318	WP_227135718.1	hypothetical protein	-
I5960_RS15795	3236272..3236655	-	384	WP_264373405.1	DUF977 family protein	-
I5960_RS15800	3236702..3237436	-	735	WP_227135720.1	replication protein P	-
I5960_RS15805	3237433..3238406	-	974	Protein_3107	replication protein	-
I5960_RS15810	3238593..3238913	-	321	WP_227135721.1	CII family transcriptional regulator	-
I5960_RS15815	3238956..3239171	-	216	WP_227135722.1	YdaS family helix-turn-helix protein	-
I5960_RS26920	3239335..3239409	+	75	Protein_3110	hypothetical protein	-
I5960_RS26925	3239623..3239973	+	351	WP_264373428.1	S24 family peptidase	-
I5960_RS15825	3240049..3240303	+	255	WP_227135724.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3225216..3260309	35093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-96)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12434.26 Da
• Isoelectric Point: 10.1211

>T182574 WP_227136875.1 NZ_CP065411:c3235744-3235421 [Kosakonia radicincitans]
MLKSIESFRDQWLKDFFLYGKATGHIPKAIETSLARKLDILNAATIVNDLRSPPGNRYENLQPPLKGYSAVRINEKYRLI
FIWKDGKATDVYLDPHSYKNKNNNHQL

Nucleotide

• Length: 324 bp

>T182574 NZ_CP086500:1907583-1907834 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12723.52 Da
• Isoelectric Point: 4.7147

>AT182574 WP_227135717.1 NZ_CP065411:c3235363-3235028 [Kosakonia radicincitans]
MKQAMRKPTTVGDVLLYEYLEPLDLKINDLAEILNVHRNSVSALVNNNRKLTTDMAFRLSVAFETSVEFWLNLQSNVDVW
EVENDARTQEELSRVVKAQSFLAEKEPKVVA

Nucleotide

• Length: 336 bp

>AT182574 NZ_CP086500:1907831-1908085 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA