Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5333286..5333866 Replicon chromosome
Accession NZ_CP065410
Organism Pseudomonas sp. IAC-BECa141

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag I5961_RS24430 Protein ID WP_085697557.1
Coordinates 5333286..5333564 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I5961_RS24435 Protein ID WP_045121630.1
Coordinates 5333579..5333866 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5961_RS24410 (I5961_24255) 5328877..5329044 - 168 WP_007950992.1 DUF2474 domain-containing protein -
I5961_RS24415 (I5961_24260) 5329103..5330110 - 1008 WP_085697554.1 cytochrome d ubiquinol oxidase subunit II -
I5961_RS24420 (I5961_24265) 5330114..5331550 - 1437 WP_085697555.1 cytochrome ubiquinol oxidase subunit I -
I5961_RS24425 (I5961_24270) 5331940..5333178 + 1239 WP_085697556.1 MFS transporter -
I5961_RS24430 (I5961_24275) 5333286..5333564 + 279 WP_085697557.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I5961_RS24435 (I5961_24280) 5333579..5333866 + 288 WP_045121630.1 HigA family addiction module antitoxin Antitoxin
I5961_RS24440 (I5961_24285) 5333872..5334423 - 552 WP_227233597.1 DJ-1/PfpI family protein -
I5961_RS24445 (I5961_24290) 5334491..5334940 - 450 WP_085732881.1 DUF4879 domain-containing protein -
I5961_RS24450 (I5961_24295) 5335161..5336456 + 1296 WP_085697559.1 NCS2 family permease -
I5961_RS24455 (I5961_24300) 5336453..5337532 + 1080 WP_227233599.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
I5961_RS24460 (I5961_24305) 5337588..5338034 - 447 WP_085697561.1 hypothetical protein -
I5961_RS24465 (I5961_24310) 5338031..5338459 - 429 WP_227233601.1 DUF4160 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-75)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10687.24 Da        Isoelectric Point: 8.9401

>T182563 WP_085697557.1 NZ_CP065410:5333286-5333564 [Pseudomonas sp. IAC-BECa141]
MIKSFQHKGLRGFYETGSTRGIRADHAKRLSRMLQFMDRAAVPGDLDLPGWRLHPLKGELCEYWSLSVSGNWRVIFRFAG
SDIELVDYLDYH

Download         Length: 279 bp

>T182563 NZ_CP086493:48375-48677 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10542.17 Da        Isoelectric Point: 6.9219

>AT182563 WP_045121630.1 NZ_CP065410:5333579-5333866 [Pseudomonas sp. IAC-BECa141]
MPMHNPPHPGETLLMDVLPELGISVTELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARMWLGVQTDYDLWQA
EHREQPAIQPIAAHA

Download         Length: 288 bp

>AT182563 NZ_CP086493:48748-49086 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References