Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 302937..303541 Replicon chromosome
Accession NZ_CP065410
Organism Pseudomonas sp. IAC-BECa141

Toxin (Protein)


Gene name higB Uniprot ID Q3KJP2
Locus tag I5961_RS01340 Protein ID WP_011331970.1
Coordinates 303227..303541 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I5961_RS01335 Protein ID WP_041475108.1
Coordinates 302937..303224 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5961_RS01310 (I5961_01310) 297972..298751 - 780 WP_227235557.1 EAL domain-containing protein -
I5961_RS01315 (I5961_01315) 298868..299275 + 408 WP_085702734.1 S4 domain-containing protein -
I5961_RS01320 (I5961_01320) 299341..300141 - 801 WP_085698009.1 phosphatase PAP2 family protein -
I5961_RS01325 (I5961_01325) 300246..301148 + 903 WP_227234121.1 Hsp33 family molecular chaperone HslO -
I5961_RS01330 (I5961_01330) 301330..302871 + 1542 WP_085702732.1 phosphoenolpyruvate carboxykinase -
I5961_RS01335 (I5961_01335) 302937..303224 - 288 WP_041475108.1 NadS family protein Antitoxin
I5961_RS01340 (I5961_01340) 303227..303541 - 315 WP_011331970.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I5961_RS01345 (I5961_01345) 303653..303952 - 300 WP_227234123.1 hypothetical protein -
I5961_RS01350 (I5961_01350) 304300..305427 - 1128 WP_085698015.1 hypothetical protein -
I5961_RS01355 (I5961_01355) 305530..305811 - 282 WP_085698016.1 hypothetical protein -
I5961_RS01360 (I5961_01360) 306116..306619 + 504 WP_227234124.1 polysaccharide deacetylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(42-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11847.76 Da        Isoelectric Point: 10.3534

>T182560 WP_011331970.1 NZ_CP065410:c303541-303227 [Pseudomonas sp. IAC-BECa141]
MIFIETRIFTRRVKELLDDDTYAAFQKQLVVSPSIGDVIEGTGGIRKTRIAAKGHGKRGGARVIYYHFVSASQIGLLMIY
PKNEQHDLSSDERKALKVLIEKWR

Download         Length: 315 bp

>T182560 NZ_CP086492:c59297-59148 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10747.32 Da        Isoelectric Point: 6.8814

>AT182560 WP_041475108.1 NZ_CP065410:c303224-302937 [Pseudomonas sp. IAC-BECa141]
MSKFFDELMESVQEMDEIVRGERTPSREFHVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAH

Download         Length: 288 bp

>AT182560 NZ_CP086492:59341-59402 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q3KJP2

Antitoxin

Source ID Structure

References