Detailed information of TA system TA182555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 66518..67218
• Replicon: plasmid pDCB1
• Accession: NZ_CP065407
• Organism: Diaphorobacter sp. JS3051

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: I3K84_RS21050
• Protein ID: WP_196996152.1
• Coordinates: 66922..67218 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: I3K84_RS21045
• Protein ID: WP_196996151.1
• Coordinates: 66518..66919 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K84_RS21020	61855..62355	-	501	WP_196996149.1	hypothetical protein	-
I3K84_RS21025	62352..63389	-	1038	WP_196996150.1	DNA-binding protein	-
I3K84_RS21030	63356..64036	-	681	WP_148262999.1	hypothetical protein	-
I3K84_RS21035	64893..65855	-	963	WP_013723326.1	replication initiator protein A	-
I3K84_RS21040	65969..66280	-	312	WP_004637272.1	putative addiction module antidote protein	-
I3K84_RS21045	66518..66919	-	402	WP_196996151.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
I3K84_RS21050	66922..67218	-	297	WP_196996152.1	type II toxin-antitoxin system MqsR family toxin	Toxin
I3K84_RS21055	67494..68529	+	1036	Protein_85	helicase/relaxase domain-containing protein	-
I3K84_RS21060	68560..69774	+	1215	WP_196996211.1	GIY-YIG nuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cap8E / bplB	1..108781	108781

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-127)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10822.40 Da
• Isoelectric Point: 6.3192

>T182555 WP_196996152.1 NZ_CP065407:c67218-66922 [Diaphorobacter sp. JS3051]
MEKRTPHCKLSAVKALVEAGNVRTTHAARTGANELGFAFADMLDVVMALTPADFYKSMTTHADHTVWQDVYHPSTQAGDV
YLKLTVIDDVLIVSFKEL

Nucleotide

• Length: 297 bp

>T182555 NZ_CP086490:c4128021-4127734 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14570.78 Da
• Isoelectric Point: 6.4555

>AT182555 WP_196996151.1 NZ_CP065407:c66919-66518 [Diaphorobacter sp. JS3051]
MKCPVCGAAELIHDTRDLPYTYKGETTVIAAVTGDFCPACAESVLDAAESNRVMREMRAFSKQVNAAIVDPGFITDVRKK
LDLDQREAAEIFGGGINAFSRYENGKTKPPLALVKLFKLLDRHPDLLNEVKAA

Nucleotide

• Length: 402 bp

>AT182555 NZ_CP086490:c4127747-4127445 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA