Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3949305..3950080 Replicon chromosome
Accession NZ_CP065406
Organism Diaphorobacter sp. JS3051

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7T1GPJ5
Locus tag I3K84_RS18770 Protein ID WP_011804662.1
Coordinates 3949622..3950080 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7T1DU90
Locus tag I3K84_RS18765 Protein ID WP_038204260.1
Coordinates 3949305..3949622 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K84_RS18745 3945190..3945402 + 213 WP_169748359.1 integrase core domain-containing protein -
I3K84_RS18750 3945427..3945750 - 324 Protein_3692 DNA-binding domain-containing protein -
I3K84_RS18755 3945901..3947325 - 1425 Protein_3693 IS5 family transposase -
I3K84_RS18760 3947423..3949006 - 1584 Protein_3694 TraI domain-containing protein -
I3K84_RS18765 3949305..3949622 + 318 WP_038204260.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
I3K84_RS18770 3949622..3950080 + 459 WP_011804662.1 type II toxin-antitoxin system YhaV family toxin Toxin
I3K84_RS18775 3950107..3950484 + 378 WP_011804663.1 DUF3742 family protein -
I3K84_RS18780 3950500..3952017 - 1518 WP_196995160.1 conjugal transfer protein TraG N-terminal domain-containing protein -
I3K84_RS18785 3952032..3952391 - 360 WP_009518522.1 hypothetical protein -
I3K84_RS18790 3952388..3953782 - 1395 WP_196995161.1 integrating conjugative element protein -
I3K84_RS18795 3953792..3954739 - 948 WP_196995162.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3923017..3985972 62955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-101)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17681.07 Da        Isoelectric Point: 10.3103

>T182553 WP_011804662.1 NZ_CP065406:3949622-3950080 [Diaphorobacter sp. JS3051]
MQRHGWTLLFHDCVIEPLQKLQAAARRAQENDPKGFESNANVKLFRALSQLMLEVVPGDPARDEYRQGNTLGSAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

>T182553 NZ_CP086490:c3786626-3786228 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11129.57 Da        Isoelectric Point: 4.3682

>AT182553 WP_038204260.1 NZ_CP065406:3949305-3949622 [Diaphorobacter sp. JS3051]
MPAIHEVATLTSKGQVTLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVQGLPED
LARAMLEHAGHGVNLDEEIDGEVEL

Download         Length: 318 bp

>AT182553 NZ_CP086490:c3786922-3786629 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1GPJ5

Antitoxin

Source ID Structure
AlphaFold DB A0A7T1DU90

References