Detailed information of TA system TA182525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 177198..177862
• Replicon: plasmid p1
• Accession: NZ_CP065398
• Organism: Bifidobacterium longum strain I2-2-3

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A395XW84
• Locus tag: I3K78_RS10905
• Protein ID: WP_032737050.1
• Coordinates: 177503..177862 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3K78_RS10900
• Protein ID: WP_007056001.1
• Coordinates: 177198..177506 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K78_RS10870 (I3K78_10860)	172528..173373	-	846	WP_212106145.1	hypothetical protein	-
I3K78_RS10875 (I3K78_10865)	173441..173917	-	477	WP_131276768.1	hypothetical protein	-
I3K78_RS10880 (I3K78_10870)	173933..174259	-	327	WP_131276765.1	hypothetical protein	-
I3K78_RS10885 (I3K78_10875)	174734..175150	-	417	WP_212106146.1	IS200/IS605 family transposase	-
I3K78_RS10890 (I3K78_10880)	175174..176460	+	1287	WP_212106147.1	RNA-guided endonuclease TnpB family protein	-
I3K78_RS10895 (I3K78_10885)	176911..177183	+	273	WP_249159921.1	hypothetical protein	-
I3K78_RS10900 (I3K78_10890)	177198..177506	-	309	WP_007056001.1	helix-turn-helix domain-containing protein	Antitoxin
I3K78_RS10905 (I3K78_10895)	177503..177862	-	360	WP_032737050.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3K78_RS10910 (I3K78_10900)	177919..178515	+	597	WP_131301871.1	hypothetical protein	-
I3K78_RS10915 (I3K78_10905)	178512..179051	+	540	WP_212106148.1	hypothetical protein	-
I3K78_RS10920 (I3K78_10910)	179410..180261	+	852	WP_212106149.1	hypothetical protein	-
I3K78_RS10925 (I3K78_10915)	180258..180758	+	501	WP_242669869.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..198121	198121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-84)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13985.16 Da
• Isoelectric Point: 10.0940

>T182525 WP_032737050.1 NZ_CP065398:c177862-177503 [Bifidobacterium longum]
MWQIEMEPIKDWLDGLDKQTAYQVYAALDLLRERGPNLKRPLVGKIEGSIIGSMKELRPGSTGRSEVRILFVFDPERKAV
LLVGGDKQNKWNKWYKTAIREAERRYIRWLEEQHGKDTK

Nucleotide

• Length: 360 bp

>T182525 NZ_CP086480:c4244122-4243772 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11911.52 Da
• Isoelectric Point: 5.2707

>AT182525 WP_007056001.1 NZ_CP065398:c177506-177198 [Bifidobacterium longum]
MSYTLEDFKADHDLDQKTIDERKEQLLEEMRLYELKEARKQQDVTQKQLAERMGVSQKRVSSLESGDVDKTEIRTLRRYL
DAIGGRLQVNAIMPDGRTLQLV

Nucleotide

• Length: 309 bp

>AT182525 NZ_CP086480:c4244367-4244116 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A395XW84