Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 73417..74081 Replicon plasmid p1
Accession NZ_CP065396
Organism Bifidobacterium longum strain K2-21-4

Toxin (Protein)


Gene name HigB2 Uniprot ID A0A395XW84
Locus tag I3K77_RS10155 Protein ID WP_032737050.1
Coordinates 73417..73776 (+) Length 120 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I3K77_RS10160 Protein ID WP_007056001.1
Coordinates 73773..74081 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K77_RS10135 (I3K77_10130) 70521..71021 - 501 WP_242669869.1 hypothetical protein -
I3K77_RS10140 (I3K77_10135) 71018..71872 - 855 WP_212110945.1 hypothetical protein -
I3K77_RS10145 (I3K77_10140) 72228..72767 - 540 WP_212106148.1 hypothetical protein -
I3K77_RS10150 (I3K77_10145) 72764..73360 - 597 WP_131301871.1 hypothetical protein -
I3K77_RS10155 (I3K77_10150) 73417..73776 + 360 WP_032737050.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I3K77_RS10160 (I3K77_10155) 73773..74081 + 309 WP_007056001.1 helix-turn-helix domain-containing protein Antitoxin
I3K77_RS10165 (I3K77_10160) 74096..74368 - 273 WP_249159921.1 hypothetical protein -
I3K77_RS10170 (I3K77_10165) 74819..76105 - 1287 WP_212106147.1 RNA-guided endonuclease TnpB family protein -
I3K77_RS10175 (I3K77_10170) 76129..76545 + 417 WP_212106146.1 IS200/IS605 family transposase -
I3K77_RS10180 (I3K77_10175) 76753..77346 + 594 WP_212110946.1 hypothetical protein -
I3K77_RS10185 (I3K77_10180) 77362..77838 + 477 WP_131276768.1 hypothetical protein -
I3K77_RS10190 (I3K77_10185) 77906..78751 + 846 WP_212106145.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..198121 198121


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(11-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-84)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13985.16 Da        Isoelectric Point: 10.0940

>T182520 WP_032737050.1 NZ_CP065396:73417-73776 [Bifidobacterium longum]
MWQIEMEPIKDWLDGLDKQTAYQVYAALDLLRERGPNLKRPLVGKIEGSIIGSMKELRPGSTGRSEVRILFVFDPERKAV
LLVGGDKQNKWNKWYKTAIREAERRYIRWLEEQHGKDTK

Download         Length: 360 bp

>T182520 NZ_CP086480:2755702-2755809 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11911.52 Da        Isoelectric Point: 5.2707

>AT182520 WP_007056001.1 NZ_CP065396:73773-74081 [Bifidobacterium longum]
MSYTLEDFKADHDLDQKTIDERKEQLLEEMRLYELKEARKQQDVTQKQLAERMGVSQKRVSSLESGDVDKTEIRTLRRYL
DAIGGRLQVNAIMPDGRTLQLV

Download         Length: 309 bp

>AT182520 NZ_CP086480:c2755655-2755589 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A395XW84

Antitoxin

Source ID Structure

References