Detailed information of TA system TA182513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1081882..1082443
• Replicon: chromosome
• Accession: NZ_CP065386
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A143CE91
• Locus tag: I5Z97_RS05275
• Protein ID: WP_005712614.1
• Coordinates: 1082165..1082443 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: I5Z97_RS05270
• Protein ID: WP_005712616.1
• Coordinates: 1081882..1082154 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Z97_RS05255	1078405..1078947	+	543	WP_021115958.1	single-stranded DNA-binding protein	-
I5Z97_RS05260	1079101..1080588	-	1488	WP_078208823.1	DUF853 domain-containing protein	-
I5Z97_RS05265	1080598..1081818	-	1221	WP_021112303.1	ATP-dependent RNA helicase RhlB	-
I5Z97_RS05270	1081882..1082154	-	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
I5Z97_RS05275	1082165..1082443	-	279	WP_005712614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5Z97_RS05280	1082592..1083602	+	1011	WP_021115969.1	ornithine carbamoyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10326.71 Da
• Isoelectric Point: 10.0505

>T182513 WP_005712614.1 NZ_CP065386:c1082443-1082165 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T182513 NZ_CP086480:1907583-1907834 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT182513 WP_005712616.1 NZ_CP065386:c1082154-1081882 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT182513 NZ_CP086480:1907831-1908085 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A143CE91