Detailed information of TA system TA182503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2521021..2521616
• Replicon: chromosome
• Accession: NZ_CP065374
• Organism: Pseudomonas aeruginosa strain PAG7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: I3251_RS11985
• Protein ID: WP_003117425.1
• Coordinates: 2521338..2521616 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3251_RS11980
• Protein ID: WP_003099268.1
• Coordinates: 2521021..2521326 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3251_RS11945 (I3251_11945)	2516170..2517018	+	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
I3251_RS11955 (I3251_11955)	2517185..2518126	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
I3251_RS11960 (I3251_11960)	2518243..2518857	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
I3251_RS11965 (I3251_11965)	2518899..2519483	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
I3251_RS11970 (I3251_11970)	2519524..2520624	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
I3251_RS11980 (I3251_11980)	2521021..2521326	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
I3251_RS11985 (I3251_11985)	2521338..2521616	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3251_RS31545	2521669..2521797	-	129	Protein_2375	integrase	-
I3251_RS11990 (I3251_11990)	2521945..2524173	+	2229	WP_003099265.1	TonB-dependent receptor	-
I3251_RS11995 (I3251_11995)	2524243..2524890	-	648	WP_003095021.1	carbonate dehydratase	-
I3251_RS12000 (I3251_12000)	2524952..2526190	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T182503 WP_003117425.1 NZ_CP065374:c2521616-2521338 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T182503 NZ_CP086475:8292-8552 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT182503 WP_003099268.1 NZ_CP065374:c2521326-2521021 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT182503 NZ_CP086475:8542-8793 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5