Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4649813..4650543 Replicon chromosome
Accession NZ_CP065358
Organism Kosakonia oryzae strain IAC-BECa90

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag I5186_RS22115 Protein ID WP_227121777.1
Coordinates 4649813..4650130 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I5186_RS22120 Protein ID WP_227121778.1
Coordinates 4650127..4650543 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5186_RS22090 (I5186_21995) 4644897..4645850 - 954 WP_064568546.1 AraC family transcriptional regulator -
I5186_RS22095 (I5186_22000) 4645969..4646874 - 906 WP_064568547.1 LysR family transcriptional regulator -
I5186_RS22100 (I5186_22005) 4646961..4647746 + 786 WP_227121775.1 NAD(P)H-dependent oxidoreductase -
I5186_RS22105 (I5186_22010) 4647937..4648509 + 573 WP_064568549.1 TetR/AcrR family transcriptional regulator -
I5186_RS22110 (I5186_22015) 4648576..4649739 + 1164 WP_227121776.1 multidrug effflux MFS transporter -
I5186_RS22115 (I5186_22020) 4649813..4650130 + 318 WP_227121777.1 type II toxin-antitoxin system HigB family toxin Toxin
I5186_RS22120 (I5186_22025) 4650127..4650543 + 417 WP_227121778.1 helix-turn-helix domain-containing protein Antitoxin
I5186_RS22125 (I5186_22030) 4650547..4651440 - 894 WP_227121779.1 MurR/RpiR family transcriptional regulator -
I5186_RS22130 (I5186_22035) 4651453..4652400 - 948 WP_227121780.1 iron-siderophore ABC transporter substrate-binding protein -
I5186_RS22135 (I5186_22040) 4652415..4653467 - 1053 WP_227121781.1 iron ABC transporter permease -
I5186_RS22140 (I5186_22045) 4653464..4654486 - 1023 WP_227121782.1 iron ABC transporter permease -
I5186_RS22145 (I5186_22050) 4654483..4655307 - 825 WP_227121783.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12533.64 Da        Isoelectric Point: 10.2933

>T182486 WP_227121777.1 NZ_CP065358:4649813-4650130 [Kosakonia oryzae]
MHLISMKAIKEAVDRFPQHKTALLTLAKTIEKADCPTPNDLKRLYPTLDNFKYLSKYYVIDIANNSLRIVALIFFENQKF
YVRHIFTHKEYDRFTEQHRSKGKKK

Download         Length: 318 bp

>T182486 NZ_CP086470:2455046-2456368 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15272.59 Da        Isoelectric Point: 4.4041

>AT182486 WP_227121778.1 NZ_CP065358:4650127-4650543 [Kosakonia oryzae]
MIVADALKATNDLIAAVPLLGEHPGEKDYQEALELVEYLLENNPTTPLLDILCAKISAYENSQPDIIALREEMAEIPPGI
AVLRTLMDQYHLTTSDFQDEIGSKSMVSRVLNGERQLNINHIKKLAARFGVSPLLFIS

Download         Length: 417 bp

>AT182486 NZ_CP086470:2454780-2455046 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References