Detailed information of TA system TA182312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1143840..1144495
• Replicon: chromosome
• Accession: NZ_CP065202
• Organism: Pseudomonas fragi strain A13BB

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I5R27_RS05080
• Protein ID: WP_196884582.1
• Coordinates: 1144151..1144495 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5R27_RS05075
• Protein ID: WP_169846206.1
• Coordinates: 1143840..1144154 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5R27_RS05060	1141714..1143114	+	1401	WP_196883902.1	FAD-binding protein	-
I5R27_RS05070	1143445..1143789	+	345	WP_134049594.1	cupin	-
I5R27_RS05075	1143840..1144154	-	315	WP_169846206.1	transcriptional regulator	Antitoxin
I5R27_RS05080	1144151..1144495	-	345	WP_196884582.1	toxin	Toxin
I5R27_RS05090	1145070..1146287	+	1218	WP_196883904.1	MFS transporter	-
I5R27_RS05095	1146368..1146892	-	525	WP_196883906.1	RNA polymerase sigma factor	-
I5R27_RS05100	1147192..1148253	+	1062	WP_196884584.1	type III secretion system gatekeeper subunit SctW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13160.86 Da
• Isoelectric Point: 6.3783

>T182312 WP_196884582.1 NZ_CP065202:c1144495-1144151 [Pseudomonas fragi]
IRTVFFETTSFTATVGDYLNDDEYRQLQVEMQANPLAGDVMPRTGGFRKLRWQDTRRGTGKRGGLRVIYYWLLDDGQFWM
FAIYDKDELENLTADQEKALKTAIGAELKQRGGK

Nucleotide

• Length: 345 bp

>T182312 NZ_CP086405:c2923691-2923588 [Enterobacter roggenkampii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11950.94 Da
• Isoelectric Point: 10.0925

>AT182312 WP_169846206.1 NZ_CP065202:c1144154-1143840 [Pseudomonas fragi]
MKKRDLFAELMQGVNEMGEQREGKITLRQYEMEALPAPEITAAEIVSIRKKLHMSQPVFARQIRTSPDTLKNWEQSKSKP
NAQAALLIKLVERFPDMVDRLNAV

Nucleotide

• Length: 315 bp

>AT182312 NZ_CP086405:2923583-2923726 [Enterobacter roggenkampii]
CAAATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT