Detailed information of TA system TA182263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24341..25013
• Replicon: chromosome
• Accession: NZ_CP065178
• Organism: Pectobacterium quasiaquaticum strain A398-S21-F17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IG605_RS00105
• Protein ID: WP_102117949.1
• Coordinates: 24627..25013 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: IG605_RS00100
• Protein ID: WP_010281167.1
• Coordinates: 24341..24640 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG605_RS00080 (IG605_000080)	19594..20583	+	990	WP_193397254.1	nucleoside hydrolase	-
IG605_RS00085 (IG605_000085)	20583..21560	+	978	WP_193407550.1	ABC transporter permease	-
IG605_RS00090 (IG605_000090)	21557..22465	+	909	WP_193407551.1	ABC transporter permease	-
IG605_RS00095 (IG605_000095)	22505..24304	+	1800	WP_193397252.1	ABC transporter ATP-binding protein	-
IG605_RS00100 (IG605_000100)	24341..24640	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
IG605_RS00105 (IG605_000105)	24627..25013	-	387	WP_102117949.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IG605_RS00110 (IG605_000110)	25335..26033	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
IG605_RS00115 (IG605_000115)	26030..26926	+	897	WP_193397251.1	2-dehydro-3-deoxygalactonokinase	-
IG605_RS00120 (IG605_000120)	26910..27527	+	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
IG605_RS00125 (IG605_000125)	27524..28672	+	1149	WP_180787230.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14837.84 Da
• Isoelectric Point: 4.7491

>T182263 WP_102117949.1 NZ_CP065178:c25013-24627 [Pectobacterium quasiaquaticum]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEILAHLSILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVSGHPFRTFFAFD
RTRKAIVLCAGDKKGEDEKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T182263 NZ_CP086370:c3155327-3155145 [Massilia sp. HC52]
ATGCTGCTCCTGATTGCTGGTGACGCGCCTTTGGGAGCGGAATGGCGAGACCATGCGTTGCGCGGTCCCTGGAGCGAGTA
TCGGGAATGCCACATAGGCGGCGACTTTCTTCTTATCTACAGAATTGCAGGGGATGTCATTACCTTTGCGCGCACAGGAA
CACATGCGGAGCTCCTTGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT182263 WP_010281167.1 NZ_CP065178:c24640-24341 [Pectobacterium quasiaquaticum]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT182263 NZ_CP086370:c3155637-3155356 [Massilia sp. HC52]
GTGCCTACTCCTGCCTCATCCATCAATATTCAGATCGATGCCGATCTGGAACGTCGCGCCAACGAAGCCCTGGCACAGGT
CGGGCTATCCATGTCGGATGCTGTCCGGATGTTTTTGCATCAAGTTGCCACCGACCAGCGGTTTCCGTGGGATCTCAGAG
TTCCAAATCAGACCACACGCGATGCAATGAAAGAATGCGACGACATAATCGCGCATCATCAAGCGCGCTTCATAAAGGCC
GAGCAACTGTTCGCAGATCTTGAAAAGAGTGGCAAAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A433N287