Detailed information of TA system TA182244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24340..25012
• Replicon: chromosome
• Accession: NZ_CP065177
• Organism: Pectobacterium quasiaquaticum strain A477-S1-J17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IG609_RS00105
• Protein ID: WP_102117949.1
• Coordinates: 24626..25012 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: IG609_RS00100
• Protein ID: WP_010281167.1
• Coordinates: 24340..24639 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG609_RS00080 (IG609_000080)	19593..20582	+	990	WP_193397254.1	nucleoside hydrolase	-
IG609_RS00085 (IG609_000085)	20582..21559	+	978	WP_010296900.1	ABC transporter permease	-
IG609_RS00090 (IG609_000090)	21556..22464	+	909	WP_193397253.1	ABC transporter permease	-
IG609_RS00095 (IG609_000095)	22504..24303	+	1800	WP_193397252.1	ABC transporter ATP-binding protein	-
IG609_RS00100 (IG609_000100)	24340..24639	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
IG609_RS00105 (IG609_000105)	24626..25012	-	387	WP_102117949.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IG609_RS00110 (IG609_000110)	25334..26032	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
IG609_RS00115 (IG609_000115)	26029..26925	+	897	WP_193400318.1	2-dehydro-3-deoxygalactonokinase	-
IG609_RS00120 (IG609_000120)	26909..27526	+	618	WP_193400319.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
IG609_RS00125 (IG609_000125)	27523..28671	+	1149	WP_180787230.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14837.84 Da
• Isoelectric Point: 4.7491

>T182244 WP_102117949.1 NZ_CP065177:c25012-24626 [Pectobacterium quasiaquaticum]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEILAHLSILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVSGHPFRTFFAFD
RTRKAIVLCAGDKKGEDEKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T182244 NZ_CP086369:2436223-2436510 [Pectobacterium versatile]
ATGAGTTATAGCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGCTCAAGCTCGATAAAACCATTCAGCAGCAATTCGC
CAAAAAACTAAAGAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAGTTACGAGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCGTCAGGCTTTCGTTTGGTTTATGAGGTTATTGACGACGTATTGATTATCGCAGTGGTAGCGGTAGGCAAA
CGTGAGCGCAGCGGTGTTTATCATCTGGCGAGTGAGCGGATGAGGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT182244 WP_010281167.1 NZ_CP065177:c24639-24340 [Pectobacterium quasiaquaticum]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT182244 NZ_CP086369:2435975-2436226 [Pectobacterium versatile]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAGCTGAAAAAGAATCCAATGGCGACGGTCAATGCTGG
TGATGGCCTGCCTGTGGCTATCTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGACCAAGAATTGGTTAAGTTAATCGGTGAACGTAAAAACCAGCCGCTGGTCGACGTGGATTTGGAT
AGTTTCCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A433N287