Detailed information of TA system TA182234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2415845..2416534
• Replicon: chromosome
• Accession: NZ_CP065172
• Organism: Klebsiella pneumoniae strain KPN110

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A331C6E2
• Locus tag: I3U25_RS11880
• Protein ID: WP_021469727.1
• Coordinates: 2415845..2416162 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3U25_RS11885
• Protein ID: WP_040147778.1
• Coordinates: 2416238..2416534 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U25_RS11850	2411547..2412056	+	510	WP_002906702.1	GNAT family N-acetyltransferase	-
I3U25_RS11855	2412066..2412992	+	927	WP_032416224.1	amino acid ABC transporter permease	-
I3U25_RS11860	2412976..2413755	+	780	WP_002906697.1	amino acid ABC transporter ATP-binding protein	-
I3U25_RS11865	2413794..2414645	+	852	WP_014343117.1	transporter substrate-binding domain-containing protein	-
I3U25_RS11870	2414723..2415340	+	618	WP_040147782.1	glutathione S-transferase family protein	-
I3U25_RS11875	2415411..2415638	+	228	WP_002906690.1	tautomerase PptA	-
I3U25_RS11880	2415845..2416162	+	318	WP_021469727.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3U25_RS11885	2416238..2416534	+	297	WP_040147778.1	helix-turn-helix domain-containing protein	Antitoxin
I3U25_RS11890	2416613..2417059	+	447	WP_004175959.1	hypothetical protein	-
I3U25_RS11895	2417100..2418716	-	1617	WP_040147777.1	carbohydrate porin	-
I3U25_RS11900	2418760..2420142	-	1383	WP_099743292.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12221.10 Da
• Isoelectric Point: 10.2217

>T182234 WP_021469727.1 NZ_CP065172:2415845-2416162 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Nucleotide

• Length: 318 bp

>T182234 NZ_CP086346:c2785860-2785570 [Clavibacter michiganensis subsp. nebraskensis]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11032.88 Da
• Isoelectric Point: 6.4721

>AT182234 WP_040147778.1 NZ_CP065172:2416238-2416534 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMPMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRQPTGAARK
LLLLLQGNPLIINLLKVV

Nucleotide

• Length: 297 bp

>AT182234 NZ_CP086346:c2786090-2785857 [Clavibacter michiganensis subsp. nebraskensis]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A331C6E2