Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 350345..350991 Replicon chromosome
Accession NZ_CP065172
Organism Klebsiella pneumoniae strain KPN110

Toxin (Protein)


Gene name higB Uniprot ID A0A332YAY3
Locus tag I3U25_RS01605 Protein ID WP_032435150.1
Coordinates 350345..350692 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag I3U25_RS01610 Protein ID WP_002920557.1
Coordinates 350692..350991 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U25_RS01595 346271..347704 + 1434 WP_002920564.1 glycogen synthase GlgA -
I3U25_RS01600 347722..350169 + 2448 WP_002920561.1 glycogen phosphorylase -
I3U25_RS01605 350345..350692 + 348 WP_032435150.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I3U25_RS01610 350692..350991 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
I3U25_RS01615 351054..352562 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
I3U25_RS01620 352767..353096 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
I3U25_RS01625 353147..353977 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
I3U25_RS01630 354027..354785 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13531.52 Da        Isoelectric Point: 5.6749

>T182224 WP_032435150.1 NZ_CP065172:350345-350692 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T182224 NZ_CP086342:c2913384-2913130 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT182224 WP_002920557.1 NZ_CP065172:350692-350991 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT182224 NZ_CP086342:c2913632-2913381 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332YAY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References