Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 337660..338246 Replicon chromosome
Accession NZ_CP065167
Organism Klebsiella pneumoniae strain KPN114

Toxin (Protein)


Gene name doc Uniprot ID A0A331ME34
Locus tag I3U24_RS01575 Protein ID WP_025861226.1
Coordinates 337878..338246 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag I3U24_RS01570 Protein ID WP_004174006.1
Coordinates 337660..337881 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U24_RS01550 333816..334742 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
I3U24_RS01555 334739..336016 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
I3U24_RS01560 336013..336780 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
I3U24_RS01565 336782..337495 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
I3U24_RS01570 337660..337881 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I3U24_RS01575 337878..338246 + 369 WP_025861226.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I3U24_RS01580 338519..339835 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
I3U24_RS01585 339942..340829 + 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
I3U24_RS01590 340826..341671 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
I3U24_RS01595 341673..342743 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 334739..343480 8741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13551.98 Da        Isoelectric Point: 8.6410

>T182203 WP_025861226.1 NZ_CP065167:337878-338246 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGIKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T182203 NZ_CP086340:2648029-2648136 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT182203 WP_004174006.1 NZ_CP065167:337660-337881 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT182203 NZ_CP086340:c2647982-2647916 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331ME34

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References