Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4141864..4142461 Replicon chromosome
Accession NZ_CP065162
Organism Klebsiella variicola strain KPN029

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag I3U23_RS20550 Protein ID WP_117106578.1
Coordinates 4142144..4142461 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I3U23_RS20545 Protein ID WP_012542525.1
Coordinates 4141864..4142151 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U23_RS20515 4137730..4137978 + 249 WP_008805539.1 DUF1158 domain-containing protein -
I3U23_RS20520 4137995..4138336 - 342 WP_117106576.1 RamA family antibiotic efflux transcriptional regulator -
I3U23_RS20525 4138376..4139482 - 1107 WP_196240918.1 MBL fold metallo-hydrolase -
I3U23_RS20530 4139662..4140243 + 582 WP_012968754.1 TetR/AcrR family transcriptional regulator -
I3U23_RS20535 4140243..4140611 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
I3U23_RS20540 4140731..4141384 + 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
I3U23_RS20545 4141864..4142151 - 288 WP_012542525.1 helix-turn-helix transcriptional regulator Antitoxin
I3U23_RS20550 4142144..4142461 - 318 WP_117106578.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I3U23_RS20555 4142646..4143689 - 1044 WP_023297181.1 hypothetical protein -
I3U23_RS20560 4144366..4145232 - 867 WP_008805530.1 helix-turn-helix transcriptional regulator -
I3U23_RS20565 4145341..4146768 + 1428 WP_040973773.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12048.34 Da        Isoelectric Point: 11.4601

>T182197 WP_117106578.1 NZ_CP065162:c4142461-4142144 [Klebsiella variicola]
IFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPASKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T182197 NZ_CP086340:1855455-1855706 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10815.51 Da        Isoelectric Point: 10.2212

>AT182197 WP_012542525.1 NZ_CP065162:c4142151-4141864 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT182197 NZ_CP086340:1855703-1855957 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References