Detailed information of TA system TA182177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 668133..668649
• Replicon: chromosome
• Accession: NZ_CP065154
• Organism: Lacticaseibacillus paracasei subsp. tolerans strain ZY-1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: I3F57_RS03255
• Protein ID: WP_003573774.1
• Coordinates: 668368..668649 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: I3F57_RS03250
• Protein ID: WP_016363783.1
• Coordinates: 668133..668375 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3F57_RS03235	663587..665758	-	2172	WP_003607130.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-
I3F57_RS03240	665930..666919	+	990	WP_003586769.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
I3F57_RS03245	666916..667401	+	486	WP_003586772.1	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	-
I3F57_RS03250	668133..668375	+	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I3F57_RS03255	668368..668649	+	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
I3F57_RS03260	668878..669417	+	540	WP_003600370.1	DUF536 domain-containing protein	-
I3F57_RS03265	669760..670443	+	684	WP_002816607.1	IS6 family transposase	-
I3F57_RS03270	670622..671950	+	1329	WP_003587117.1	glycoside-pentoside-hexuronide (GPH):cation symporter	-
I3F57_RS03275	672151..672708	+	558	WP_003567312.1	hypothetical protein	-
I3F57_RS03280	672986..673225	+	240	Protein_651	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	565571..684080	118509
	-	flank	IS/Tn	-	-	662006..663421	1415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T182177 WP_003573774.1 NZ_CP065154:668368-668649 [Lacticaseibacillus paracasei subsp. tolerans]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T182177 NZ_CP086338:2129977-2130153 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT182177 WP_016363783.1 NZ_CP065154:668133-668375 [Lacticaseibacillus paracasei subsp. tolerans]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT182177 NZ_CP086338:2130199-2130615 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7