Detailed information of TA system TA182170

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 165507..165932
• Replicon: plasmid unnamed1
• Accession: NZ_CP065153
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: H7994_RS24755
• Protein ID: WP_001332444.1
• Coordinates: 165507..165668 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	165721..165932 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS24725	160857..161084	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
H7994_RS24730	161178..161864	-	687	WP_072646475.1	PAS domain-containing protein	-
H7994_RS24735	162054..162437	-	384	WP_000124981.1	relaxosome protein TraM	-
H7994_RS24740	162771..163361	+	591	WP_171947368.1	transglycosylase SLT domain-containing protein	-
H7994_RS24745	163657..164478	-	822	WP_072646476.1	DUF945 domain-containing protein	-
H7994_RS24750	164657..165269	+	613	Protein_175	hypothetical protein	-
H7994_RS24755	165507..165668	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	165721..165932	-	212	NuclAT_0	-	Antitoxin
-	165721..165932	-	212	NuclAT_0	-	Antitoxin
-	165721..165932	-	212	NuclAT_0	-	Antitoxin
-	165721..165932	-	212	NuclAT_0	-	Antitoxin
H7994_RS24760	165943..166662	-	720	WP_072646477.1	plasmid SOS inhibition protein A	-
H7994_RS24765	166659..167093	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H7994_RS24770	167148..169106	-	1959	WP_072646478.1	ParB/RepB/Spo0J family partition protein	-
H7994_RS24775	169165..169398	-	234	WP_001209608.1	DUF905 domain-containing protein	-
H7994_RS24780	169461..169958	-	498	WP_050427786.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA / iroB / iroC	1..237102	237102
	-	flank	IS/Tn	-	-	170804..171307	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T182170 WP_001332444.1 NZ_CP065153:c165668-165507 [Escherichia coli O150:H6]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T182170 NZ_CP086338:390604-390954 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 212 bp

>AT182170 NZ_CP065153:c165932-165721 [Escherichia coli O150:H6]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin