Detailed information of TA system TA182167

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94908..95162
• Replicon: plasmid unnamed1
• Accession: NZ_CP065153
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H7994_RS24380
• Protein ID: WP_001351576.1
• Coordinates: 94908..95114 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	95101..95162 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS24345	90452..90799	+	348	Protein_94	IS1-like element IS1A family transposase	-
H7994_RS24350	91239..91526	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H7994_RS24355	91523..91774	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H7994_RS24360	92737..93593	-	857	Protein_97	incFII family plasmid replication initiator RepA	-
H7994_RS24365	93586..94068	-	483	WP_001273588.1	hypothetical protein	-
H7994_RS24370	94061..94135	-	75	WP_001442103.1	RepA leader peptide Tap	-
H7994_RS24375	94367..94624	-	258	WP_000083845.1	replication regulatory protein RepA	-
H7994_RS24380	94908..95114	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	95101..95162	+	62	NuclAT_1	-	Antitoxin
-	95101..95162	+	62	NuclAT_1	-	Antitoxin
-	95101..95162	+	62	NuclAT_1	-	Antitoxin
-	95101..95162	+	62	NuclAT_1	-	Antitoxin
H7994_RS24385	95405..96241	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
H7994_RS24390	96241..97044	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
H7994_RS24395	97151..98274	-	1124	Protein_104	IS3-like element IS1133 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA / iroB / iroC	1..237102	237102
	-	inside	IScluster/Tn	aph(6)-Id / aph(3'')-Ib / blaTEM-1B / tet(A) / aph(3')-IIa	-	87084..122647	35563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T182167 WP_001351576.1 NZ_CP065153:c95114-94908 [Escherichia coli O150:H6]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T182167 NZ_CP086336:3966487-3966951 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT182167 NZ_CP065153:95101-95162 [Escherichia coli O150:H6]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin