Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3755012..3755574 Replicon chromosome
Accession NZ_CP065152
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag H7994_RS18045 Protein ID WP_000605675.1
Coordinates 3755296..3755574 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H7994_RS18040 Protein ID WP_000781370.1
Coordinates 3755012..3755296 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS18025 3750193..3753240 + 3048 WP_196315986.1 formate dehydrogenase-N subunit alpha -
H7994_RS18030 3753253..3754137 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
H7994_RS18035 3754130..3754783 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
H7994_RS18040 3755012..3755296 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
H7994_RS18045 3755296..3755574 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7994_RS18050 3755760..3756770 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
H7994_RS18055 3756904..3758601 - 1698 WP_000433464.1 malate dehydrogenase -
H7994_RS18060 3758758..3758895 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H7994_RS18065 3758997..3759212 - 216 WP_000495766.1 biofilm-dependent modulation protein -
H7994_RS18070 3759557..3759988 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T182154 WP_000605675.1 NZ_CP065152:c3755574-3755296 [Escherichia coli O150:H6]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T182154 NZ_CP086336:c1916164-1916057 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT182154 WP_000781370.1 NZ_CP065152:c3755296-3755012 [Escherichia coli O150:H6]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT182154 NZ_CP086336:1916211-1916277 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References