Detailed information of TA system TA182147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2497031..2497725
• Replicon: chromosome
• Accession: NZ_CP065152
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: H7994_RS11905
• Protein ID: WP_001263500.1
• Coordinates: 2497327..2497725 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7994_RS11900
• Protein ID: WP_000554758.1
• Coordinates: 2497031..2497324 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS11875	2492468..2492965	+	498	Protein_2308	REP-associated tyrosine transposase RayT	-
H7994_RS11880	2493147..2493305	-	159	WP_157848283.1	hypothetical protein	-
H7994_RS11885	2493384..2495096	-	1713	Protein_2310	flagellar biosynthesis protein FlhA	-
H7994_RS11890	2495068..2495853	+	786	WP_061336152.1	putative lateral flagellar export/assembly protein LafU	-
H7994_RS11895	2495924..2496979	+	1056	WP_001226168.1	DNA polymerase IV	-
H7994_RS11900	2497031..2497324	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7994_RS11905	2497327..2497725	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7994_RS11910	2497735..2498187	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
H7994_RS11915	2498377..2499516	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
H7994_RS11920	2499513..2500127	+	615	WP_000602123.1	peptide chain release factor H	-
H7994_RS11925	2500184..2501641	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
H7994_RS11930	2501902..2502360	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2475731..2498187	22456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T182147 WP_001263500.1 NZ_CP065152:2497327-2497725 [Escherichia coli O150:H6]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T182147 NZ_CP086334:4566990-4567301 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT182147 WP_000554758.1 NZ_CP065152:2497031-2497324 [Escherichia coli O150:H6]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT182147 NZ_CP086334:4567298-4567717 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3