Detailed information of TA system TA182143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1557790..1558392
• Replicon: chromosome
• Accession: NZ_CP065152
• Organism: Escherichia coli O150:H6 strain FEX669

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: H7994_RS07510
• Protein ID: WP_000897302.1
• Coordinates: 1557790..1558101 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7994_RS07515
• Protein ID: WP_000356397.1
• Coordinates: 1558102..1558392 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7994_RS07480	1552820..1553605	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
H7994_RS07485	1553704..1554303	+	600	WP_001314324.1	glucose-1-phosphatase	-
H7994_RS07490	1554297..1555169	+	873	WP_000920762.1	virulence factor BrkB family protein	-
H7994_RS07495	1555166..1555603	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7994_RS07500	1555648..1556589	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7994_RS07505	1556653..1557561	-	909	WP_001331553.1	alpha/beta hydrolase	-
H7994_RS07510	1557790..1558101	+	312	WP_000897302.1	hypothetical protein	Toxin
H7994_RS07515	1558102..1558392	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7994_RS07520	1558477..1558689	+	213	WP_000197774.1	hypothetical protein	-
H7994_RS07525	1558751..1559029	+	279	WP_001296612.1	hypothetical protein	-
H7994_RS07530	1559425..1559643	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
H7994_RS07535	1559867..1560796	-	930	WP_089699770.1	formate dehydrogenase accessory protein FdhE	-
H7994_RS07540	1560793..1561428	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7994_RS07545	1561425..1562327	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T182143 WP_000897302.1 NZ_CP065152:1557790-1558101 [Escherichia coli O150:H6]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T182143 NZ_CP086334:4145283-4145579 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT182143 WP_000356397.1 NZ_CP065152:1558102-1558392 [Escherichia coli O150:H6]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT182143 NZ_CP086334:4145581-4145976 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: S1P416