Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 121755..122482 Replicon chromosome
Accession NZ_CP065152
Organism Escherichia coli O150:H6 strain FEX669

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag H7994_RS00750 Protein ID WP_000547563.1
Coordinates 122171..122482 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H7994_RS00745 Protein ID WP_000126304.1
Coordinates 121755..122174 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7994_RS00730 117918..120170 - 2253 WP_012601682.1 carbamoyltransferase HypF -
H7994_RS00735 120323..120850 - 528 WP_001078777.1 electron transport protein HydN -
H7994_RS00740 121235..121663 + 429 WP_000536064.1 hypothetical protein -
H7994_RS00745 121755..122174 - 420 WP_000126304.1 helix-turn-helix domain-containing protein Antitoxin
H7994_RS00750 122171..122482 - 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
H7994_RS00755 122645..123400 - 756 WP_001553332.1 hypothetical protein -
H7994_RS00760 123443..123913 - 471 WP_196316078.1 hydrogenase maturation peptidase HycI -
H7994_RS00765 123906..124316 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
H7994_RS00770 124313..125080 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
H7994_RS00775 125080..125622 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
H7994_RS00780 125632..127341 - 1710 WP_001288136.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T182138 WP_000547563.1 NZ_CP065152:c122482-122171 [Escherichia coli O150:H6]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T182138 NZ_CP086334:3583830-3583937 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15474.50 Da        Isoelectric Point: 4.4596

>AT182138 WP_000126304.1 NZ_CP065152:c122174-121755 [Escherichia coli O150:H6]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT182138 NZ_CP086334:c3583772-3583718 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure

References