Detailed information of TA system TA182094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4164656..4165350
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: I2T83_RS20090
• Protein ID: WP_001521903.1
• Coordinates: 4164656..4165054 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I2T83_RS20095
• Protein ID: WP_000554758.1
• Coordinates: 4165057..4165350 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS20060	4159826..4161070	-	1245	WP_000189541.1	esterase FrsA	-
-	4160486..4160566	-	81	NuclAT_11	-	-
-	4160486..4160566	-	81	NuclAT_11	-	-
-	4160486..4160566	-	81	NuclAT_11	-	-
-	4160486..4160566	-	81	NuclAT_11	-	-
I2T83_RS20065	4161162..4161620	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
I2T83_RS20070	4161881..4163338	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
I2T83_RS20075	4163395..4163747	-	353	Protein_3934	peptide chain release factor H	-
I2T83_RS20080	4163743..4163949	-	207	Protein_3935	RtcB family protein	-
I2T83_RS20085	4164194..4164646	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
I2T83_RS20090	4164656..4165054	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I2T83_RS20095	4165057..4165350	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I2T83_RS20100	4165402..4166457	-	1056	WP_001226177.1	DNA polymerase IV	-
I2T83_RS20105	4166528..4167313	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
I2T83_RS20110	4167285..4168997	+	1713	Protein_3941	flagellar biosynthesis protein FlhA	-
I2T83_RS20115	4169076..4169429	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
I2T83_RS20120	4169486..4170235	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T182094 WP_001521903.1 NZ_CP065136:c4165054-4164656 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T182094 NZ_CP086305:c1087620-1087282 [Burkholderia ambifaria]
ATGGTTGACATGCTGCGATCCATGCCATACAGTGCACCTACATTCAGTATCCGGACCACCGACGTCTTCGACGCGTGGTT
TGCCGGCCTGCAGGATCGCGTCGCGAAGCGCCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTGTCGATCACGGGCCCGGCTATCGCGTCTACTACGTCAGGCGCGGA
ACGATATGGGTGATCCTGCTCTGCGGCGGCGACAAGTCGACGCAACAGGCGGACATCCGCGCCGCGCACGCGATGCTCGC
GCGGCTCGACCTGGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT182094 WP_000554758.1 NZ_CP065136:c4165350-4165057 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT182094 NZ_CP086305:c1087278-1086979 [Burkholderia ambifaria]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGACGACGACATGGCCGACTACTTCGAGGC
CTGTCTGCAGGAAGCCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGCGCGCGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCATGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAATTGCACGGCGCGAAGGCGCACGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3