Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2057882..2058384 Replicon chromosome
Accession NZ_CP065136
Organism Escherichia coli strain EE72d

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I2T83_RS09800 Protein ID WP_000767829.1
Coordinates 2058130..2058384 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I2T83_RS09795 Protein ID WP_001259255.1
Coordinates 2057882..2058133 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2T83_RS09770 2053060..2054127 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I2T83_RS09775 2054127..2055197 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
I2T83_RS09780 2055194..2056498 - 1305 WP_021523144.1 histidinol dehydrogenase -
I2T83_RS09785 2056504..2057403 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
I2T83_RS09790 2057549..2057599 - 51 WP_001364200.1 his operon leader peptide -
I2T83_RS09795 2057882..2058133 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I2T83_RS09800 2058130..2058384 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I2T83_RS09805 2058467..2059291 + 825 WP_000754758.1 SDR family oxidoreductase -
I2T83_RS09810 2059337..2060266 + 930 WP_023154044.1 LysR family transcriptional regulator -
I2T83_RS09815 2060481..2060543 + 63 WP_010723108.1 membrane protein YoeI -
I2T83_RS09820 2060533..2061891 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I2T83_RS09825 2062070..2063128 + 1059 WP_000492324.1 transport protein YeeE -
I2T83_RS09830 2063142..2063369 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T182078 WP_000767829.1 NZ_CP065136:2058130-2058384 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T182078 NZ_CP086298:1939416-1939697 [Burkholderia ambifaria]
ATGACCTTTGAGCTTGCCTTTCTCGAACCTGCGTTGAAGGAGTGGAAGAAGCTCGATCGCACCATCCGCGACCAGTTCAA
ATCCCGACTCGCCGAGCGCCTCGAGAATCCCCGCATTGCGTCGGCCAAGCTGCATGGCCATCCGGATCGCTACAAGATCA
AGCTGCGCAGCGTCGGCTACCGACTCGTCTACGAAGTGCGCGATACGGAAGTGATCGTACTGGTTGTCGCAGTCGGTCGC
CGTGACCGCGATGCCGTCTATCTTGCCGCGATGAAGCGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT182078 WP_001259255.1 NZ_CP065136:2057882-2058133 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT182078 NZ_CP086298:1939178-1939426 [Burkholderia ambifaria]
ATGCCTCACACCATTCTGGCCAATGTTACGGCGAGCGTTTCGGAACTGAAGCGCAATCCGATGGGAACCGTCGCGGCGGG
CGAGGGCTTTCCGGTTGCGATTCTGAATCACAACGAGCCCGCGTTCTATTGCATCCCGGCGAAGACGTGGGAAGCGATGG
TCGAACGTCTCGAAGATATAGAAGACAACGCGCTTGCCAATACGCGTGCGAAGGACAAGGTCGTCAAGGTCAAGCTGAAT
GACCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References