Detailed information of TA system TA182077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1253888..1254615
• Replicon: chromosome
• Accession: NZ_CP065136
• Organism: Escherichia coli strain EE72d

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: I2T83_RS06005
• Protein ID: WP_001521139.1
• Coordinates: 1253888..1254199 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I2T83_RS06010
• Protein ID: WP_000126294.1
• Coordinates: 1254196..1254615 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I2T83_RS05980	1249823..1251532	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
I2T83_RS05985	1251542..1252084	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I2T83_RS05990	1252084..1252851	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I2T83_RS05995	1252848..1253258	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I2T83_RS06000	1253251..1253721	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I2T83_RS06005	1253888..1254199	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
I2T83_RS06010	1254196..1254615	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I2T83_RS06015	1254694..1256118	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
I2T83_RS06020	1256127..1257584	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I2T83_RS06025	1257844..1258854	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
I2T83_RS06030	1259003..1259530	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T182077 WP_001521139.1 NZ_CP065136:1253888-1254199 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T182077 NZ_CP086298:1171975-1172274 [Burkholderia ambifaria]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGACGACGACATGGCCGACTACTTCGAGGC
CTGTCTGCAGGAAGCCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAGGCGCACGCATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT182077 WP_000126294.1 NZ_CP065136:1254196-1254615 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT182077 NZ_CP086298:1171654-1171971 [Burkholderia ambifaria]
ATGCCATACAGTGCACCTGCATTCAGTATCCGGACCACCGACGTCTTCGACGCGTGGTTTGCCGGCCTGCAGGATCGCGT
CGCGAAGCGCCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACTGGAAATCCGCCGGCTCCCCCG
TCGTCGAAATGCGTATCGATCACGGGCCCGGCTATCGCGTCTACTACGTCAGGCGCGGAACGATATGGGTGATCCTGCTC
TGCGGCGGCGACAAGTCGACGCAACAGGCGGACATCCGCGCCGCGCACGCGATGCTCGCGCGGCTCGACCTGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9