Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 628392..629191 Replicon chromosome
Accession NZ_CP065136
Organism Escherichia coli strain EE72d

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag I2T83_RS02975 Protein ID WP_000347272.1
Coordinates 628392..628856 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag I2T83_RS02980 Protein ID WP_001307405.1
Coordinates 628856..629191 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I2T83_RS02945 623393..623827 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
I2T83_RS02950 623845..624723 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
I2T83_RS02955 624713..625492 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
I2T83_RS02960 625503..625976 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I2T83_RS02965 625999..627279 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
I2T83_RS02970 627528..628337 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I2T83_RS02975 628392..628856 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I2T83_RS02980 628856..629191 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I2T83_RS02985 629340..630911 - 1572 WP_001273738.1 galactarate dehydratase -
I2T83_RS02990 631286..632620 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I2T83_RS02995 632636..633406 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T182071 WP_000347272.1 NZ_CP065136:c628856-628392 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T182071 NZ_CP086297:1768802-1769224 [Burkholderia ambifaria]
GTGACGACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCAGCCGCGGTGTTGTCCCGGTT
GCAGTCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCGATCACCTATGCCGAGATGCGGTTCGGCGCAGTCGGCA
GAAAGGCTTCGCCGAAGCATCCGGAACTCGTGACGGCCTTTGTCTCGCGGCTGGATGGCGTGCTGTCGTGGGATACCGCG
GCAGTCGATGCAACGACCGCGATTCGCGTCGACCTTGCCGCACGTGGCACGCCGATCGGCGCGAACGACGCGTCGATCGC
CGGTCATGCGATCGCGGCCGGTGCCGTGCTTGTAACGAACAACAGTCGCGAATTCAGCCGTGTCGCGGGGTTGTCGCTCG
AAGACTGGGCAGCCTCGGCCTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT182071 WP_001307405.1 NZ_CP065136:c629191-628856 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT182071 NZ_CP086297:1768539-1768805 [Burkholderia ambifaria]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGTGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGTTGCTGCTGCGTCCGGTGCGGCCGACGTGGCTGTCGTTCGCGACTGAAC
CACGGGCCGATGCCGACTTCCTCGTCGAGCGCCCGACGGTGGTCGAAGCCGGTCGTTTCGACCTGCCCGGCGCCGATGAC
GATGCACCGACGGAGTCGGCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References