Detailed information of TA system TA182018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4124331..4124839
• Replicon: chromosome
• Accession: NZ_CP065037
• Organism: Leifsonia sp. ZF2019

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: IT072_RS20100
• Protein ID: WP_223358605.1
• Coordinates: 4124579..4124839 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: IT072_RS20095
• Protein ID: WP_223358604.1
• Coordinates: 4124331..4124582 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT072_RS20065 (IT072_19965)	4120167..4121064	+	898	Protein_3945	IS5 family transposase	-
IT072_RS20070 (IT072_19970)	4121084..4121458	-	375	WP_223358599.1	EXLDI protein	-
IT072_RS20075 (IT072_19975)	4121638..4122048	-	411	WP_223358600.1	DUF1214 domain-containing protein	-
IT072_RS20080 (IT072_19980)	4122320..4122601	+	282	WP_223358601.1	flagellar FlbD family protein	-
IT072_RS20085 (IT072_19985)	4122617..4123435	+	819	WP_223358602.1	MotA/TolQ/ExbB proton channel family protein	-
IT072_RS20090 (IT072_19990)	4123437..4124267	+	831	WP_223358603.1	flagellar motor protein MotB	-
IT072_RS20095 (IT072_19995)	4124331..4124582	+	252	WP_223358604.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
IT072_RS20100 (IT072_20000)	4124579..4124839	+	261	WP_223358605.1	Txe/YoeB family addiction module toxin	Toxin
IT072_RS20105 (IT072_20005)	4124864..4125613	-	750	WP_223358606.1	ABC transporter permease	-
IT072_RS20110 (IT072_20010)	4125623..4126417	-	795	WP_223358607.1	AraC family transcriptional regulator	-
IT072_RS20115 (IT072_20015)	4126467..4126907	+	441	WP_223358608.1	DUF2000 family protein	-
IT072_RS20120 (IT072_20020)	4127204..4128526	+	1323	WP_223358609.1	sodium:proton exchanger	-
IT072_RS20125 (IT072_20025)	4128721..4129113	-	393	WP_223358610.1	PIN domain-containing protein	-
IT072_RS20130 (IT072_20030)	4129110..4129382	-	273	WP_223358611.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10316.81 Da
• Isoelectric Point: 9.4417

>T182018 WP_223358605.1 NZ_CP065037:4124579-4124839 [Leifsonia sp. ZF2019]
MKLIWDQNAWEDYLYWQAQDRRVVKRINQLLVDILRNGNTGIGKPEALKHGFQGFWSRRITDEHRLVYKVTEDAVLIAQC
RYHYAP

Nucleotide

• Length: 261 bp

>T182018 NZ_CP086259:4776830-4777207 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.47 Da
• Isoelectric Point: 4.3423

>AT182018 WP_223358604.1 NZ_CP065037:4124331-4124582 [Leifsonia sp. ZF2019]
MKTLSYSESRAHYAATLDAVLDDREEVVITRAGREPVVMVALEDYESLKETAYLLRSPANARRLIESIERLDDGEGTPHD
LLE

Nucleotide

• Length: 252 bp

>AT182018 NZ_CP086259:4776406-4776783 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA