Detailed information of TA system TA182005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 57242..57867
• Replicon: chromosome
• Accession: NZ_CP065036
• Organism: Pectobacterium parmentieri strain PB20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3FNM7
• Locus tag: PB20LOC_RS00380
• Protein ID: WP_033070820.1
• Coordinates: 57242..57547 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PB20LOC_RS00385
• Protein ID: WP_103808699.1
• Coordinates: 57544..57867 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PB20LOC_RS00355	52964..53611	+	648	WP_012822190.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
PB20LOC_RS00360	53639..54556	+	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
PB20LOC_RS00365	54557..54850	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
PB20LOC_RS00370	56385..56756	-	372	WP_103808702.1	helix-turn-helix domain-containing protein	-
PB20LOC_RS00375	56830..57090	-	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PB20LOC_RS00380	57242..57547	+	306	WP_033070820.1	type II toxin-antitoxin system HigB family toxin	Toxin
PB20LOC_RS00385	57544..57867	+	324	WP_103808699.1	helix-turn-helix domain-containing protein	Antitoxin
PB20LOC_RS00390	59030..59488	+	459	WP_012822197.1	hypothetical protein	-
PB20LOC_RS00395	59569..60921	-	1353	WP_103808643.1	aspartate aminotransferase family protein	-
PB20LOC_RS00400	60952..62223	-	1272	WP_103808673.1	MFS transporter	-
PB20LOC_RS00405	62378..62824	+	447	WP_012822200.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-105)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12010.78 Da
• Isoelectric Point: 10.0386

>T182005 WP_033070820.1 NZ_CP065036:57242-57547 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Nucleotide

• Length: 306 bp

>T182005 NZ_CP086259:1949412-1949666 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11655.44 Da
• Isoelectric Point: 5.0877

>AT182005 WP_103808699.1 NZ_CP065036:57544-57867 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDAPEFVAFNERIKALPAGIALLRILMDQHGLTQSDFEAEIGKKSLVSRIL
NGERTLTVDHMSALAKRFDIPVSAFVD

Nucleotide

• Length: 324 bp

>AT182005 NZ_CP086259:1949164-1949415 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A8B3FNM7