Detailed information of TA system TA181952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 25454..26126
• Replicon: chromosome
• Accession: NZ_CP065031
• Organism: Pectobacterium brasiliense strain F126

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F126LOC_RS00115
• Protein ID: WP_119870773.1
• Coordinates: 25740..26126 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A433N287
• Locus tag: F126LOC_RS00110
• Protein ID: WP_010281167.1
• Coordinates: 25454..25753 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F126LOC_RS00090	20719..21696	+	978	WP_119870771.1	nucleoside hydrolase	-
F126LOC_RS00095	21696..22673	+	978	WP_010296900.1	ABC transporter permease	-
F126LOC_RS00100	22670..23578	+	909	WP_039275990.1	ABC transporter permease	-
F126LOC_RS00105	23618..25417	+	1800	WP_119870772.1	ABC transporter ATP-binding protein	-
F126LOC_RS00110	25454..25753	-	300	WP_010281167.1	XRE family transcriptional regulator	Antitoxin
F126LOC_RS00115	25740..26126	-	387	WP_119870773.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F126LOC_RS00120	26448..27146	+	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
F126LOC_RS00125	27143..28039	+	897	WP_119870774.1	2-dehydro-3-deoxygalactonokinase	-
F126LOC_RS00130	28023..28640	+	618	WP_010281152.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
F126LOC_RS00135	28637..29785	+	1149	WP_010281150.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14718.77 Da
• Isoelectric Point: 5.0357

>T181952 WP_119870773.1 NZ_CP065031:c26126-25740 [Pectobacterium brasiliense]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEVLAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGENAKLFYERMIKIADAEFASHLISPEVKEDGDA

Nucleotide

• Length: 387 bp

>T181952 NZ_CP086220:c1948807-1948556 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11048.08 Da
• Isoelectric Point: 10.6442

>AT181952 WP_010281167.1 NZ_CP065031:c25753-25454 [Pectobacterium brasiliense]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT181952 NZ_CP086220:c1948559-1948305 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A433N287