Detailed information of TA system TA181946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1706208..1706868
• Replicon: chromosome
• Accession: NZ_CP065030
• Organism: Pectobacterium versatile strain F131

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A221T809
• Locus tag: F131LOC_RS07600
• Protein ID: WP_044203475.1
• Coordinates: 1706208..1706561 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F131LOC_RS07605
• Protein ID: WP_010298722.1
• Coordinates: 1706566..1706868 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F131LOC_RS07580	1702521..1703777	+	1257	WP_103971203.1	multidrug effflux MFS transporter	-
F131LOC_RS07585	1703823..1704662	-	840	WP_044203470.1	glutathione-dependent disulfide-bond oxidoreductase	-
F131LOC_RS07590	1704827..1705438	-	612	WP_044203472.1	NAD(P)-dependent oxidoreductase	-
F131LOC_RS07595	1705541..1705981	+	441	WP_044203474.1	helix-turn-helix transcriptional regulator	-
F131LOC_RS07600	1706208..1706561	+	354	WP_044203475.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F131LOC_RS07605	1706566..1706868	+	303	WP_010298722.1	XRE family transcriptional regulator	Antitoxin
F131LOC_RS07610	1706906..1707910	-	1005	WP_103971202.1	threonine/serine dehydratase	-
F131LOC_RS07615	1707963..1708313	+	351	WP_103971201.1	helix-turn-helix domain-containing protein	-
F131LOC_RS07620	1708341..1708733	-	393	WP_103971234.1	helix-turn-helix transcriptional regulator	-
F131LOC_RS07625	1708823..1709248	+	426	WP_039540880.1	DoxX family protein	-
F131LOC_RS07630	1709368..1710003	+	636	WP_103971200.1	NAD(P)H-binding protein	-
F131LOC_RS07635	1710071..1710664	-	594	WP_103862008.1	flavodoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13609.59 Da
• Isoelectric Point: 9.8031

>T181946 WP_044203475.1 NZ_CP065030:1706208-1706561 [Pectobacterium versatile]
VWRIETTDVFDSWFSALNDRDRACVLAALIVLREKGPMLSRPYADTVKGSRYSNMKELRIQSRGTPIRAFFAFDPHRTGI
LLCAGNKSGNEKRFYDEMLPFAERELTNYLKKSEKRE

Nucleotide

• Length: 354 bp

>T181946 NZ_CP086220:c1153084-1152890 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11282.16 Da
• Isoelectric Point: 5.6573

>AT181946 WP_010298722.1 NZ_CP065030:1706566-1706868 [Pectobacterium versatile]
MGRTLEQLLADEKPEVVVDAHAIATEMLLNIHLAELRERVQKTQVEMAQVLGLKQPTVAVMEKPGRDLKLSTLKRYVEAT
GSKLRLDVELPDGSHYEFML

Nucleotide

• Length: 303 bp

>AT181946 NZ_CP086220:1152713-1152891 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A221T809