Detailed information of TA system TA181939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 398277..399001
• Replicon: chromosome
• Accession: NZ_CP065030
• Organism: Pectobacterium versatile strain F131

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F131LOC_RS01770
• Protein ID: WP_103971372.1
• Coordinates: 398277..398588 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F131LOC_RS01775
• Protein ID: WP_103861592.1
• Coordinates: 398585..399001 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F131LOC_RS01740	393290..393937	+	648	WP_010308671.1	type I-E CRISPR-associated protein Cse2/CasB	-
F131LOC_RS01745	393941..395008	+	1068	WP_103971369.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
F131LOC_RS01750	395018..395752	+	735	WP_103971370.1	type I-E CRISPR-associated protein Cas5/CasD	-
F131LOC_RS01755	395752..396399	+	648	WP_095701367.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
F131LOC_RS01760	396427..397344	+	918	WP_010308688.1	type I-E CRISPR-associated endonuclease Cas1	-
F131LOC_RS01765	397345..397638	+	294	WP_103971371.1	type I-E CRISPR-associated endoribonuclease Cas2	-
F131LOC_RS01770	398277..398588	+	312	WP_103971372.1	type II toxin-antitoxin system HigB family toxin	Toxin
F131LOC_RS01775	398585..399001	+	417	WP_103861592.1	helix-turn-helix domain-containing protein	Antitoxin
F131LOC_RS01780	399208..399711	-	504	WP_103861591.1	hypothetical protein	-
F131LOC_RS01785	400058..400294	+	237	WP_103971373.1	type I-E CRISPR-associated endoribonuclease Cas2	-
F131LOC_RS01790	401200..401658	+	459	WP_010286715.1	hypothetical protein	-
F131LOC_RS01795	401756..403108	-	1353	WP_103971374.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12460.33 Da
• Isoelectric Point: 9.8530

>T181939 WP_103971372.1 NZ_CP065030:398277-398588 [Pectobacterium versatile]
MHVIPRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T181939 NZ_CP086215:2771097-2771306 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15405.70 Da
• Isoelectric Point: 4.8037

>AT181939 WP_103861592.1 NZ_CP065030:398585-399001 [Pectobacterium versatile]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT181939 NZ_CP086215:2770792-2771085 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA