Detailed information of TA system TA181916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1123760..1124378
• Replicon: chromosome
• Accession: NZ_CP065020
• Organism: Sinorhizobium meliloti strain S35m

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: I0J99_RS05300
• Protein ID: WP_010969011.1
• Coordinates: 1124082..1124378 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I0J99_RS05295
• Protein ID: WP_010969010.1
• Coordinates: 1123760..1124068 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I0J99_RS05265	1119430..1120507	-	1078	Protein_1033	ISAs1 family transposase	-
I0J99_RS05270	1120755..1121096	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
I0J99_RS05275	1121681..1121947	+	267	WP_003527312.1	sel1 repeat family protein	-
I0J99_RS05280	1121969..1123090	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
I0J99_RS05290	1123519..1123749	-	231	WP_026029805.1	hypothetical protein	-
I0J99_RS05295	1123760..1124068	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
I0J99_RS05300	1124082..1124378	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I0J99_RS05305	1124426..1124731	-	306	WP_127590582.1	hypothetical protein	-
I0J99_RS05310	1125059..1126096	+	1038	WP_003527304.1	porin	-
I0J99_RS05315	1126206..1127273	-	1068	WP_014526650.1	site-specific integrase	-
I0J99_RS05320	1127314..1128363	-	1050	WP_010969013.1	porin	-
I0J99_RS05325	1128577..1129065	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1119430..1120194	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T181916 WP_010969011.1 NZ_CP065020:c1124378-1124082 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T181916 NZ_CP086203:c3788679-3788332 [Morganella morganii]
ATGGCCTATTATGCATTATCGACAGGTGTTATTACCTGTTCACAGGCGGCGGGACGCTTCAGACGTAATATGCCTCCGGA
AATTCCGGTTATTCTGCCGGGACGTCTTGCTGTTGATGAAAGCGTAAAAGGCAGAGGGATCGGACGGGGTCTGATAAAAG
ATGCCGCACTGCGGGTCTTGCAGGCAGCCGGGATTGTGGGTATCCGCGGTATTGTTGTCCGTGCTCTGTCTGATAATGCC
AGACGTTTTTATGAGCATACCGGTTTTATACCGTCACCGGCTGACCCGATGCTGCTGATGATTACATTACGGGATCTGCA
GCTGGCAACCGGTATCTATTCTGAATAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT181916 WP_010969010.1 NZ_CP065020:c1124068-1123760 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT181916 NZ_CP086203:c3788984-3788679 [Morganella morganii]
ATGACAAAACACATAGATGAGCAGCATCCTGTGTCCGGCGTGAATATCACCCGTTCAACACTGAATTTACGCATCAAACC
GGAAGATAAAATTCTGATTGATCGCGCAGCGCATGCTGCCGGTAAAAACAGAACCGAGTTCGTACTGGAAGCTGCGCGGC
GTGCCGCCGAAGAAACACTGGCAGATTTACGGGTTATTGATGTCAGTACCGATGTCTATCAGCAGTTTATTACCAGACTG
GATATGGCACCACAGTCCGGTGAGGCGCTTCACGGACATTTGTCGTCTGTCACCATAACCGGATAA

Structures

Toxin

• AlphaFold DB: Q92R61