Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 829846..830457 Replicon plasmid pBS3d
Accession NZ_CP064935
Organism Rhizobium phaseoli strain BS3

Toxin (Protein)


Gene name graT Uniprot ID A0A192TNT1
Locus tag HER27_RS31295 Protein ID WP_037113455.1
Coordinates 829846..830127 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A246TMY6
Locus tag HER27_RS31300 Protein ID WP_037144436.1
Coordinates 830158..830457 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HER27_RS31275 825404..826813 - 1410 WP_064820400.1 aldehyde dehydrogenase family protein -
HER27_RS31280 826810..827874 - 1065 WP_064820401.1 homoserine dehydrogenase -
HER27_RS31285 828108..828575 + 468 WP_004670133.1 Lrp/AsnC family transcriptional regulator -
HER27_RS31290 829047..829337 - 291 WP_037113457.1 hypothetical protein -
HER27_RS31295 829846..830127 + 282 WP_037113455.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HER27_RS31300 830158..830457 + 300 WP_037144436.1 HigA family addiction module antidote protein Antitoxin
HER27_RS31305 830718..831611 - 894 WP_004670117.1 amidohydrolase -
HER27_RS31310 831676..832653 - 978 WP_064820402.1 substrate-binding domain-containing protein -
HER27_RS31315 832694..833704 - 1011 WP_037113453.1 ABC transporter permease -
HER27_RS31320 833701..835203 - 1503 WP_004670111.1 sugar ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1111454 1111454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-80)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10455.94 Da        Isoelectric Point: 9.1204

>T181902 WP_037113455.1 NZ_CP064935:829846-830127 [Rhizobium phaseoli]
VIRSFKDAKTEAVARGKAPKGFPSDIVRSAIRKLTILNSVAVLEDLRSPPGNRLEALKGNREGQHSIRINDQWRICFVWT
DAGIEDVEIVDYH

Download         Length: 282 bp

>T181902 NZ_CP086201:46026-46415 [Enterobacter roggenkampii]
ATGCTGGATACGAATATTGTGAGTCACTTGTTTAAACGACACCCGGAGGTTGTAAGCAGAATGACATGTCTGGCGCCGGG
TGACGTTTGTATCTCAAGCATTACCGAAGCGGAATTGCTGTATGGTGCTGACAAGAAGAAAAGCAGTAGATTGAAAGAAA
CAATCCGGGAGTTTCTCAATACCATCACGATTTGCGACTGGGACAGCGACGCCGCAGCAACGTACGGTGAACTCCGTGCA
ACCCTGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAACTGATCGCTGCACATGCTATCAGTCGGGGAACAACGAT
CGTTACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAACGTTGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11344.19 Da        Isoelectric Point: 6.2263

>AT181902 WP_037144436.1 NZ_CP064935:830158-830457 [Rhizobium phaseoli]
MTHILPPVHPGEILREEYLLPLNMSAGALAKKLNVPRTRIERIAAEQTPITTDTALRLAKYFRTTPEFWMNMQTSFNLKT
EEMALHSELELIPEIDRAA

Download         Length: 300 bp

>AT181902 NZ_CP086201:45754-46020 [Enterobacter roggenkampii]
ATGGAACGCACAGCAAAACTATTCAGAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGCGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TNT1

Antitoxin

Source ID Structure
AlphaFold DB A0A246TMY6

References