Detailed information of TA system TA181834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4271402..4272201
• Replicon: chromosome
• Accession: NZ_CP064847
• Organism: Escherichia coli strain 20

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: IPT86_RS20565
• Protein ID: WP_000347273.1
• Coordinates: 4271737..4272201 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: IPT86_RS20560
• Protein ID: WP_001308975.1
• Coordinates: 4271402..4271737 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPT86_RS20545	4267187..4267957	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
IPT86_RS20550	4267973..4269307	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
IPT86_RS20555	4269682..4271253	+	1572	WP_001273755.1	galactarate dehydratase	-
IPT86_RS20560	4271402..4271737	+	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IPT86_RS20565	4271737..4272201	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IPT86_RS20570	4272256..4273065	-	810	WP_000072180.1	aga operon transcriptional regulator AgaR	-
IPT86_RS20575	4273314..4274594	+	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IPT86_RS20580	4274617..4275090	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IPT86_RS20585	4275101..4275880	+	780	WP_075862220.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
IPT86_RS20590	4275870..4276748	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
IPT86_RS20595	4276766..4277200	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4260920..4272201	11281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T181834 WP_000347273.1 NZ_CP064847:4271737-4272201 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T181834 NZ_CP086183:464060-464341 [Xanthomonas oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT181834 WP_001308975.1 NZ_CP064847:4271402-4271737 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT181834 NZ_CP086183:464359-464649 [Xanthomonas oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08