Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2437732..2438294 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag IPT86_RS11905 Protein ID WP_000605676.1
Coordinates 2437732..2438010 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag IPT86_RS11910 Protein ID WP_000781370.1
Coordinates 2438010..2438294 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS11880 2433318..2433749 - 432 WP_000152305.1 peroxiredoxin OsmC -
IPT86_RS11885 2434094..2434309 + 216 WP_000495766.1 biofilm-dependent modulation protein -
IPT86_RS11890 2434411..2434548 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
IPT86_RS11895 2434705..2436402 + 1698 WP_000433464.1 malate dehydrogenase -
IPT86_RS11900 2436536..2437546 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
IPT86_RS11905 2437732..2438010 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IPT86_RS11910 2438010..2438294 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
IPT86_RS11915 2438345..2438998 - 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
IPT86_RS11920 2438991..2439875 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
IPT86_RS11925 2439888..2442935 - 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T181830 WP_000605676.1 NZ_CP064847:2437732-2438010 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T181830 NZ_CP086182:3576912-3577241 [Xanthomonas oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT181830 WP_000781370.1 NZ_CP064847:2438010-2438294 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT181830 NZ_CP086182:3577238-3577657 [Xanthomonas oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References