Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1173360..1174039 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag IPT86_RS05730 Protein ID WP_000057541.1
Coordinates 1173360..1173662 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag IPT86_RS05735 Protein ID WP_000806442.1
Coordinates 1173698..1174039 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS05710 1168623..1169843 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
IPT86_RS05715 1170061..1171713 + 1653 WP_000771723.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
IPT86_RS05720 1171750..1172229 - 480 WP_000186626.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
IPT86_RS05725 1172433..1173227 - 795 WP_000365157.1 TraB/GumN family protein -
IPT86_RS05730 1173360..1173662 + 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IPT86_RS05735 1173698..1174039 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
IPT86_RS05740 1174097..1176601 - 2505 WP_061092784.1 copper-exporting P-type ATPase CopA -
IPT86_RS05745 1176864..1177796 + 933 WP_000883039.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T181820 WP_000057541.1 NZ_CP064847:1173360-1173662 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T181820 NZ_CP086180:3734126-3735049 [Xanthomonas oryzae]
ATGAACGAACCTGACAAGAGCAGGCGCACTGTTGCCGCGTCGGTTCGCGCCCGGCTGCTCAACAAGGCCCGCGCCGAAAG
ACTCGACTTCAACCTGCTGCTGACCCGCTACGCGCTGGAGCGCGTCCTTTACCGCCTGAGCGTTTCGGAGCAGCAGGGTC
AATTCTTGCTCAAAGGTGCGCTGCTGTTCGACATCTGGTTCGACATACCGCATCGCCCGACGCACGATGCCGACCTGCTG
GGCTTCGGCTCCACCGAGATCCCACATCTGGAAGACCTGTTCCGCAGAATCAGTCAAATCGCCAGCGATGACGGCATCGT
GTTTCAGGCCGACAGCGTGAAGGCCGCCGAAATCCGCAAGGAGGCCAACTACGCGGGCGTCCGTGTGACGATGATCGGCC
TGCTCGATGGCGCGCGCTGCACGGTGCAGATCGACATCGGTTTCGGCGACGCCGTGACGCCCGGCCCGGACAACGTGCAG
TACCCAGTCATCCTCGACGGCATGCCGCAGCCGCAACTGCGGGTCTATCCCCGCTACACCGTGGTCGCCGAAAAGCTGGA
AGCGATGGTCAAGCTCGGCATCCTCAACAGCCGGATGAAGGACTACTTCGACCTCTGGGTTCTCGCCGGACATTCCGACT
TCGACGGTGCGGTGCTGGCCACGGCCATTCGCGCCACGTTCGAGTGCAGGGGTACGGCCATCCCACCCGGCGCGCCGCTG
GGCCTCACCGATGAGTTCGGCCTGGACGAGCAGAAGACAAAGCAGTGGCAGGCGTTCCTGCGCAAGAACACCTTGGAGCC
GATGCCGCTGACCACGGTGATCGAAGCGCTGCGCGAATTCGTGCTGCCCGTGCTGGCCGCGCTCGCGGCAGGCGAAGGTT
TCGACCGCCAGTGGCGCGCGGGCGCGGGATGGGATGCGGCCTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT181820 WP_000806442.1 NZ_CP064847:1173698-1174039 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT181820 NZ_CP086180:3733530-3734129 [Xanthomonas oryzae]
ATGGACACGACCGTCGACAAGCTGCTGGAGCTGGTGCGCACGCGGGGACTTCTGCGCCCGCACGATCTGGCCCCACTGGG
CATCCCACGGGTGGCCCTGACGCGGGCCGTTCGGCGCGGGCAACTGGAGCGCGTCGGGCGCGGCCTGTACGGCCTGGTGG
CGCGTCCGGTGTCGGCCCACGGCACGCTGGCCGAAGTCGCGCGCCGCGTGCCCAAGGGCGTGGTCTGCCTGCTGTCGGCG
CTGCGCGTCCACGACCTGACGACCCAGGCCCCGTTCGAGGTGTGGCTGGCCATCGACAACAAGGCCGCTGCCCCGAAGCT
GGACTACCCGCCGCTACGTCTCGTGCGCTTCTCCGGGCCGGCGCTGAGCGAGGGCGTCGAGGAACACGTTGTTGATGGTG
TGCCCGTCCACGTGACCAGCGTCGCCAAGACCGTGGCCGACTGCTTCAAGTACCGCAACAAGATCGGCATCGACGTGGCG
CTGGAAGCGCTGCGCGAGGCGTGGGTTGCCCGGCGCATGACCAGCGACGAGATCTGGCACTACGCCAAGATCGACCGCGT
CGCCAACGTGATGCGTCCCTATCTGGAGAGCCTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References