Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 903517..904211 Replicon chromosome
Accession NZ_CP064847
Organism Escherichia coli strain 20

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag IPT86_RS04335 Protein ID WP_001263491.1
Coordinates 903813..904211 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag IPT86_RS04330 Protein ID WP_000554755.1
Coordinates 903517..903810 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IPT86_RS04310 899261..899758 + 498 WP_000006244.1 transposase -
IPT86_RS04315 899870..901582 - 1713 Protein_835 flagellar biosynthesis protein FlhA -
IPT86_RS04320 901554..902339 + 786 WP_179145761.1 putative lateral flagellar export/assembly protein LafU -
IPT86_RS04325 902410..903465 + 1056 WP_075862061.1 DNA polymerase IV -
IPT86_RS04330 903517..903810 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IPT86_RS04335 903813..904211 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IPT86_RS04340 904221..904673 + 453 WP_075862062.1 GNAT family N-acetyltransferase -
IPT86_RS04345 904864..906003 + 1140 WP_170990260.1 RNA ligase RtcB family protein -
IPT86_RS04350 906000..906614 + 615 WP_075862064.1 peptide chain release factor H -
IPT86_RS04355 906676..907185 + 510 WP_001361775.1 hydrolase -
IPT86_RS04360 907202..907444 - 243 WP_001029361.1 hypothetical protein -
IPT86_RS04365 907492..908949 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T181817 WP_001263491.1 NZ_CP064847:903813-904211 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T181817 NZ_CP086180:c1764185-1764051 [Xanthomonas oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT181817 WP_000554755.1 NZ_CP064847:903517-903810 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT181817 NZ_CP086180:c1764401-1764249 [Xanthomonas oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References