Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | /Xre-PIN |
| Location | 9713..10373 | Replicon | plasmid pSYSG |
| Accession | NC_005231 | ||
| Organism | Synechocystis sp. PCC 6803 (Synechocystis PCC6803) | ||
Toxin (Protein)
| Gene name | - | Uniprot ID | - |
| Locus tag | slr8014 | Protein ID | WP_011153785.1 |
| Coordinates | 9948..10373 (+) | Length | 142 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | - |
| Locus tag | ssr8013 | Protein ID | WP_011153784.1 |
| Coordinates | 9713..9940 (+) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SGL_RS01160 (sll8007) | 6043..6393 | - | 351 | WP_011153778.1 | DUF5615 family PIN-like protein | - |
| SGL_RS01165 (ssl8008) | 6377..6601 | - | 225 | WP_011153779.1 | DUF433 domain-containing protein | - |
| SGL_RS01170 (sll8009) | 6652..8619 | - | 1968 | Protein_9 | SAM-dependent DNA methyltransferase | - |
| SGL_RS01180 (sll8011) | 8821..9195 | - | 375 | WP_011153782.1 | DUF5615 family PIN-like protein | - |
| SGL_RS01185 (sll8012) | 9205..9621 | - | 417 | WP_011153783.1 | hypothetical protein | - |
| SGL_RS01190 (ssr8013) | 9713..9940 | + | 228 | WP_011153784.1 | DUF2281 domain-containing protein | Antitoxin |
| SGL_RS01195 (slr8014) | 9948..10373 | + | 426 | WP_011153785.1 | PIN domain-containing protein | Toxin |
| SGL_RS01200 (slr8015) | 10395..10994 | + | 600 | WP_011153786.1 | ParA family protein | - |
| SGL_RS01205 (slr8016) | 10998..11867 | + | 870 | WP_011153787.1 | ParB/RepB/Spo0J family partition protein | - |
| SGL_RS01215 (sll8017) | 12618..13151 | - | 534 | WP_011153788.1 | hypothetical protein | - |
| SGL_RS01220 (sll8018) | 13695..15287 | - | 1593 | WP_011153789.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | - | - | 1..44343 | 44343 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 142 a.a. Molecular weight: 15962.62 Da Isoelectric Point: 4.3429
>T1818 WP_011153785.1 NC_005231:9948-10373 [Synechocystis sp. PCC 6803]
VKVLLDTNIVLDLLLEREPFIEETIAIFEEIEQGNIEAYIAATTVTNIFYIIRKNKSREVALIAVKRLLIGLQLCPVNLQ
VIEVALDINLRDFEDSVQLACATVNQLTAIITRDTKDFVESPLPIYSPAECLYRVKTNKLD
VKVLLDTNIVLDLLLEREPFIEETIAIFEEIEQGNIEAYIAATTVTNIFYIIRKNKSREVALIAVKRLLIGLQLCPVNLQ
VIEVALDINLRDFEDSVQLACATVNQLTAIITRDTKDFVESPLPIYSPAECLYRVKTNKLD
Download Length: 426 bp
>T1818 NC_005231:9948-10373 [Synechocystis sp. PCC 6803]
GTGAAAGTACTTCTCGACACCAATATTGTTCTCGACTTACTTTTAGAGAGAGAACCGTTTATCGAAGAAACCATAGCCAT
CTTTGAAGAAATCGAGCAGGGAAACATCGAAGCTTACATTGCGGCTACAACAGTTACCAATATTTTTTACATCATTCGTA
AGAACAAAAGCCGCGAGGTGGCGCTTATAGCTGTTAAGCGACTACTGATTGGACTACAACTATGCCCCGTGAATCTACAA
GTTATCGAAGTTGCCCTCGACATCAATCTAAGAGACTTTGAAGATAGTGTCCAATTGGCATGTGCCACAGTTAACCAACT
TACTGCAATCATTACCCGTGACACCAAAGACTTTGTTGAGAGTCCTTTGCCTATTTACTCCCCTGCGGAATGTTTATATC
GGGTTAAGACCAATAAGCTTGATTAG
GTGAAAGTACTTCTCGACACCAATATTGTTCTCGACTTACTTTTAGAGAGAGAACCGTTTATCGAAGAAACCATAGCCAT
CTTTGAAGAAATCGAGCAGGGAAACATCGAAGCTTACATTGCGGCTACAACAGTTACCAATATTTTTTACATCATTCGTA
AGAACAAAAGCCGCGAGGTGGCGCTTATAGCTGTTAAGCGACTACTGATTGGACTACAACTATGCCCCGTGAATCTACAA
GTTATCGAAGTTGCCCTCGACATCAATCTAAGAGACTTTGAAGATAGTGTCCAATTGGCATGTGCCACAGTTAACCAACT
TACTGCAATCATTACCCGTGACACCAAAGACTTTGTTGAGAGTCCTTTGCCTATTTACTCCCCTGCGGAATGTTTATATC
GGGTTAAGACCAATAAGCTTGATTAG
Antitoxin
Download Length: 76 a.a. Molecular weight: 8420.71 Da Isoelectric Point: 6.2748
>AT1818 WP_011153784.1 NC_005231:9713-9940 [Synechocystis sp. PCC 6803]
MTAQEKLYQLIQTLPEKEVSEILNFAESLWPKVSSPKIPAGTLTGLRGIAKPTGPVPSDAELQRQYTDYLKEKYL
MTAQEKLYQLIQTLPEKEVSEILNFAESLWPKVSSPKIPAGTLTGLRGIAKPTGPVPSDAELQRQYTDYLKEKYL
Download Length: 228 bp
>AT1818 NC_005231:9713-9940 [Synechocystis sp. PCC 6803]
ATGACAGCGCAAGAAAAACTCTACCAACTAATTCAAACTCTGCCAGAGAAGGAAGTTAGCGAAATACTCAATTTTGCCGA
GTCGCTTTGGCCAAAAGTCTCTAGCCCCAAAATTCCCGCCGGCACCCTTACCGGACTGCGGGGCATTGCCAAACCAACGG
GCCCCGTACCTAGCGACGCTGAGTTACAAAGGCAATACACTGACTACCTAAAAGAGAAGTATCTGTAA
ATGACAGCGCAAGAAAAACTCTACCAACTAATTCAAACTCTGCCAGAGAAGGAAGTTAGCGAAATACTCAATTTTGCCGA
GTCGCTTTGGCCAAAAGTCTCTAGCCCCAAAATTCCCGCCGGCACCCTTACCGGACTGCGGGGCATTGCCAAACCAACGG
GCCCCGTACCTAGCGACGCTGAGTTACAAAGGCAATACACTGACTACCTAAAAGAGAAGTATCTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Stefan Kopfmann et al. (2016) Type II Toxin-Antitoxin Systems in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803. Toxins 8(7):228. [PubMed:27455323]