Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 284004..284698 Replicon chromosome
Accession NZ_CP064825
Organism Escherichia coli UTI89

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag IIE09_RS01320 Protein ID WP_001263500.1
Coordinates 284300..284698 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag IIE09_RS01315 Protein ID WP_000554758.1
Coordinates 284004..284297 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IIE09_RS01290 279540..280037 + 498 WP_000006241.1 REP-associated tyrosine transposase RayT -
IIE09_RS01295 280120..280278 - 159 WP_014639450.1 hypothetical protein -
IIE09_RS01300 280357..282069 - 1713 Protein_252 flagellar biosynthesis protein FlhA -
IIE09_RS01305 282041..282826 + 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
IIE09_RS01310 282897..283952 + 1056 WP_001226168.1 DNA polymerase IV -
IIE09_RS01315 284004..284297 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IIE09_RS01320 284300..284698 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IIE09_RS01325 284708..285160 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
IIE09_RS01330 285350..286489 + 1140 WP_000521577.1 RNA ligase RtcB family protein -
IIE09_RS01335 286486..287100 + 615 WP_000602123.1 peptide chain release factor H -
IIE09_RS01340 287157..288614 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
IIE09_RS01345 288875..289333 + 459 WP_001291988.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 264479..285160 20681


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T181763 WP_001263500.1 NZ_CP064825:284300-284698 [Escherichia coli UTI89]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T181763 NZ_CP086128:1857185-1857287 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT181763 WP_000554758.1 NZ_CP064825:284004-284297 [Escherichia coli UTI89]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT181763 NZ_CP086128:c1857294-1857149 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References