Detailed information of TA system TA181756

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrG-as-bsrG/-
• Location: 112770..112934
• Replicon: plasmid p_unnamed
• Accession: NZ_CP064819
• Organism: Bacillus subtilis strain SH1

Toxin (Protein)

• Gene name: bsrG
• Uniprot ID: L8EAY0
• Locus tag: ITP52_RS22085
• Protein ID: WP_009967548.1
• Coordinates: 112770..112886 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	as-bsrG
Locus tag	-
Coordinates	112881..112934 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITP52_RS22045	108226..108618	+	393	WP_019712881.1	hypothetical protein	-
ITP52_RS22050	108639..108890	+	252	WP_019712880.1	phage holin	-
ITP52_RS22055	108942..109835	+	894	Protein_152	tetratricopeptide repeat protein	-
ITP52_RS22060	109825..110001	+	177	WP_019712878.1	hypothetical protein	-
ITP52_RS22065	109998..111272	-	1275	WP_128472254.1	Y-family DNA polymerase	-
ITP52_RS22070	111265..111597	-	333	WP_109962767.1	YolD-like family protein	-
ITP52_RS22075	111771..112106	+	336	WP_041054825.1	hypothetical protein	-
ITP52_RS22080	112150..112509	-	360	WP_041338710.1	hypothetical protein	-
ITP52_RS22085	112770..112886	+	117	WP_009967548.1	type I toxin-antitoxin system toxin BsrG	Toxin
-	112881..112934	-	54	NuclAT_1	-	Antitoxin
-	112881..112934	-	54	NuclAT_1	-	Antitoxin
-	112881..112934	-	54	NuclAT_1	-	Antitoxin
-	112881..112934	-	54	NuclAT_1	-	Antitoxin
ITP52_RS22090	112978..113436	-	459	WP_109962768.1	SMI1/KNR4 family protein	-
ITP52_RS22095	113449..115335	-	1887	WP_109962769.1	HNH endonuclease	-
ITP52_RS22100	115468..116001	-	534	WP_109962770.1	SMI1/KNR4 family protein	-
ITP52_RS22105	116086..116336	-	251	Protein_162	PH domain-containing protein	-
ITP52_RS22110	116516..117400	+	885	WP_109962771.1	endonuclease YokF	-
ITP52_RS22115	117443..117916	-	474	WP_109962772.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118493	118493

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-38)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4336.39 Da
• Isoelectric Point: 10.1022

>T181756 WP_009967548.1 NZ_CP064819:112770-112886 [Bacillus subtilis]
MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK

Nucleotide

• Length: 117 bp

>T181756 NZ_CP086122:c4717812-4717465 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Nucleotide

• Length: 54 bp

>AT181756 NZ_CP064819:c112934-112881 [Bacillus subtilis]
AAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAAACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7Z7EVU9

Antitoxin