Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3965327..3965924 Replicon chromosome
Accession NZ_CP064768
Organism Klebsiella pneumoniae subsp. pneumoniae strain HS20R14

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HS20R14_RS19305 Protein ID WP_004142563.1
Coordinates 3965607..3965924 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HS20R14_RS19300 Protein ID WP_004142561.1
Coordinates 3965327..3965614 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS20R14_RS19270 (3961407) 3961407..3961655 + 249 WP_020325243.1 DUF1158 domain-containing protein -
HS20R14_RS19275 (3961673) 3961673..3962014 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
HS20R14_RS19280 (3962045) 3962045..3963160 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
HS20R14_RS19285 (3963340) 3963340..3963921 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
HS20R14_RS19290 (3963921) 3963921..3964289 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
HS20R14_RS19295 (3964409) 3964409..3965062 + 654 WP_196547702.1 oxygen-insensitive NAD(P)H nitroreductase -
HS20R14_RS19300 (3965327) 3965327..3965614 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HS20R14_RS19305 (3965607) 3965607..3965924 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HS20R14_RS19310 (3966109) 3966109..3967152 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
HS20R14_RS19315 (3967822) 3967822..3968688 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HS20R14_RS19320 (3968797) 3968797..3970224 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T181684 WP_004142563.1 NZ_CP064768:c3965924-3965607 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T181684 NZ_CP086069:c2654847-2654278 [Rhodococcus pyridinivorans]
ATGAGGCGTCTGGATCTGATCGTCGGTCCCAACGGTTCCGGCAAGTCGACGTTCGTCGAGTTGTCCCTCGCTCCCCTGCT
GCCGCGCAGCGTGTTCGTCAATGCGGATGTGATCGCGCGGCAGCGCTGGCCCGACCGGGCGGAGGAGATGTCGTACGAGG
CGGCGCAACTCGCCGCGGCGGCGCGGGACCGGTTGCTGCGGCAGGGCCGGTCGTTCATTGCGGAGACGGTGTTCTCGCAT
CCGTCGAAGCTGGAGTTGATCGACCGCGCGCACGAGGCGGGGTTCACGGTGGTGCTGCACGTGATGCTGGTGCCCGAGGC
GATGTCGGTGCTGCGTGTACAGCACCGGGTGGCCTCCGGTGGGCATGCGGTGCCCGAGGACAAGATCCGGGCGCGCTATC
GGCGGTTGTGGCCGCTGGTGACCGAAGCGATCGGCCGCGCCGATCAGGCGTCGGTGTACGACACGGCGTCCGGGACCCCG
GTGGTGGTGGCGCGGTTCGCCGGCGGGGTGCCGGTGGGTGCGGTGCGCTGGCCGGGATGGGCGCCGGCGGCGCTCACCGG
GCTCGGCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT181684 WP_004142561.1 NZ_CP064768:c3965614-3965327 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT181684 NZ_CP086069:c2655230-2654844 [Rhodococcus pyridinivorans]
ATGACGTCATCCACCGACAAGGTCACCCGCTTCGACGCCGAACTCGTCGACAGCGCCATCGCCGAGGGCGGCCGCCAGAA
CCGCACCGGACGCCAGCAACTCGAATACTGGGCCCGCATCGGCCGCGCGATGACCGCCCACGAGACCGCCTCCCTGCACC
GGGTGCACGAGGCGCTGGCCGGGACACGGGAGCTGAGCGAGCTCACCGCCGCCGAGGGACGGCTGTTCGACGCGGAGATC
GATGCGCGCCTGGCGGGCGGGCTGGCCCGCACCGACTACGCCGAGGTTCTCGCCGCGCGTGGGGTCACCACGGTGGTGCT
CGACGACGAAGGCCGGCTGGTCGAGCAGCGTCCCGACGGTAGTCGCCGGGTGCTCGACGACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References